Toxicogenimics Laboratory Core

这个实验室核心的目标是提供基础设施， 和核心专业知识的项目1-4，以实现其目标。伯克利超级基金的总体目标 基础研究计划（SBRP）是应用功能基因组学，蛋白质组学，转录组学， 纳米技术，以更好地检测砷，汞，苯，多环芳烃， 三氯乙烯和其他超级基金优先化学品的环境;评估其影响， 人类健康，特别是儿童等易受影响人群的健康; 降低其毒性。项目1至4将使用功能基因组学，蛋白质组学， 转录组学在他们的研究中。此外，项目1和3是流行病学研究， 复杂的样品处理，使这些技术可以应用。项目成功1- 4在很大程度上取决于生物样本的有效处理和管理，以及获取 以及最新的“-omic”技术。因此，详细的收集和存储协议 将为细胞遗传学、基因分型、基因表达和 项目1-4中提出的蛋白质组分析。核心C的具体目标是：1）处理，维护 并储存生物样品和细胞系; 2）提供细胞遗传学的设施和方法 分析; 3）提供使用Affytek、Illumina和定制阵列进行基因表达谱分析的设施 技术; 4）提供使用各种质谱分析的蛋白质组学分析设施 技术; 5）提供遗传多态性分析的设施和方法， 使用ABI 7900序列检测系统的基于Taqman的微球阵列技术， Illumina Bead Station平台。在所有需要的领域都获得了专门知识，沿着 核心D的计算生物学技能，应该允许成功应用“组学”技术 在伯克利SBRP的项目1-4。

The goal of this laboratory core is to provide the infrastructure and core expertise for Projects 1-4 to achieve their goals. The overall goal of the Berkeley Superfund Basic Research Program (SBRP) is to apply functional genomics, proteomics, transcriptomics, and nanotechnology to better detect arsenic, mercury, benzene, polycyclic aromatic hydrocarbons, trichloroethylene, and other Superfund priority chemicals in the environment; evaluate their effects on human health, especially the health of susceptible populations such as children; and remediate their presence and reduce their toxicity. Projects 1 through 4 will use functional genomics, proteomics, and transcriptomics in their studies. Further, Projects 1 and 3 are epidemiological studies that require sophisticated sample processing so that these technologies can be applied. The success of Projects 1- 4 largely depends on the effective handling and management of biological samples, as well as access to and expertise in the latest "-omic" technologies. Thus, detailed collection and storage protocols have been designed and core facilities will be provided for the cytogenetic, genotyping, gene expression and proteomic analyses proposed in Projects 1-4. The specific aims of Core C are to: 1) Process, maintain and store biological samples and cell lines; 2) provide facilities and methodologies for cytogenetic analysis; 3) provide facilities for gene expression profiling using Affymetrix, Illumina, and custom array technologies; 4) provide facilities for proteomic analyses using various mass spectrometric technologies; and, 5) provide facilities and methodologies for the analysis of genetic polymorphisms by Taqman-based and bead array technologies using the ABI 7900 Sequence Detection System and Illumina Bead Station platforms. Expertise in all required areas has been attained that, along with the computational biology skills of Core D, should allow the successful application of "-omic" technologies in Projects 1-4 of the Berkeley SBRP.