Immunology/Molecular Biology Core

核心B将集中管理所有免疫学和分子生物学 支持组成秘鲁TMRC的两个科学项目所需的程序，从而优化 利用资源，保持严格的质量控制标准。CORE的具体目标 B负责(1)协调与免疫学和分子生物学程序有关的所有工作 秘鲁TMRC的研究项目和流行病学/地理信息系统/数据管理核心；(2) 协调和监督所有生物标本的收集和处理程序；(3) 标准化、执行和监测所有免疫和分子生物学程序；(4)优化 实验室人员、设备和用品的使用；(5)协调登记和整理 分析结果；(5)提供有效转让和传播所获得技术的手段； (6)为大学生提供免疫学和寄生虫学培训的环境， 访问调查员。主要程序包括血清学和寄生虫学检测 人、豚鼠和狗的克氏毛滴虫感染的诊断(上鞭虫体抗原-EL ISA和 免疫荧光抗体检测、异种诊断、血液培养)、使用类鞭毛虫 排泄分泌抗原(TESA)在标准的Western印迹、ELISA、猎鹰试验筛查试验中- ELISA和一种新的现场友好的快速TESA-印迹方法，使用预先准备的试条，允许快速转向 围绕着一系列T.ruzi分子技术(以核DNA卫星为靶标的检测PCR 重复序列或动质DNA小环、核剪接先导序列用于克氏锥虫离散分类 分型单位及其与兰氏锥虫、克鲁兹锥虫DNA RAPDS的区别)，微卫星座位 致病黑色素瘤与表面增强激光解吸电离飞行时间 生物标志物鉴定的光谱分析。所有的医疗点免疫测试，快速- 将在我们的现场进行酶联免疫吸附试验和快速现场友好形式的TESA印迹， 玻利维亚的阿雷基帕和圣克鲁斯。PCR和RT-PCR也将在UPCH进行 实验室，然后这项技术将转移到玻利维亚。新技术，如变种 加州大学洛杉矶分校将开发使用多位点分型的分型，然后将其转移到UPCH 实验室。致病旋毛虫的微卫星基因座检测将在 寄生虫病昆虫学实验室。人血、动物血和T。 感染动物的粪便和豚鼠的异种诊断都将在阿雷基帕或阿雷基帕现场进行。 圣克鲁斯。所有蛋白质组学工作将在英国伦敦帝国理工学院进行。这个 这些程序的实施将支持两个研究项目的科学工作 并通过与秘鲁科学家的交流为秘鲁科学家提供重要的培训机会 美国、英国和巴西的合作者和顾问。 表现。

Core B will centralize the management of all of the immunology and molecular biology procedures neededto support the two scientific projects that form the Peru TMRC, thus optimizing the use of resources and maintaining rigorousquality control standards. The specific aims of Core B are to (1) coordinate all work related to immunology and molecular biology procedures between both Research Projects and the Epidemiology/GIS/Data Management core of the Peru TMRC; (2) coordinate and oversee collection and handling procedures for all biological specimens; (3) standardize, perform, and monitor all immunological and molecular biology procedures; (4) optimize the use of laboratory personnel, equipment and supplies; (5) coordinate the register and collation of assay results; (5) provide the means for an efficient transfer and diffusion of acquired technology; (6) provide the environment for immunology and parasitology training for University students and visiting investigators. The major procedures consist of serologic and parasitologic assays for the diagnosis of T.cruzi infection in humans, guinea pigs and dogs (epimastigote antigen ELISA and immunofluoresecent antibody tests, xenodiagnosis, hemoculture), the use of Trypomastigote excreted-secreted antigens (TESA) in standard Western blot, ELISA, Falcon assay screening test - ELISA and a novel field-friendly rapid TESA-blot using pre-prepared strips to allow rapid turn- around, a range of T.cruzi molecular techniques (Detection PCR targeting nuclear DNA satellite repeats or kinetoplast DNA minicircles, Nuclear spliced leader array for classifying T. cruzi discrete typing units' and distinguishing it from T.ranglei, T.cruzi DNA RAPDS), microsatellite loci for Triatoma infestans and Surface-enhanced laser desorption ionization-time of flight mass spectrometry for the indentification of biomarkers. All point-of-care immunological tests, FAST- ELISA and the rapid field-friendly format of the TESA blot will be conducted in our field sites, Arequipa and Santa Cruz, Bolivia. PCRand RT- PCR will also be performed at the UPCH laboratory and then the technique will be transferred to Bolivia. New techniques such as strain typing using multi - locus typing will be developed at UCLA andthen transferred to the UPCH laboratory. Microsatellite loci testing for T. infestans will be performed at the CDC Division of Parasitic Diseases entomology laboratory. Hemoculture of blood from humans, animals and T. infestans feces, and xenodiagnosis in guinea pigs, will all be performed on site either in Arequipa or Santa Cruz. All proteomics work will be conducted at the Imperial College, London, UK. The implementation of these procedures will support the scientific work of the two Research Projects and provide significant training opportunities for Peruvian scientists through interchanges with the US, UK and Brazilian collaborators and consultants. PERFORMANCE.