DETERMINANTS OF CKS SUBSTRATE SPECIFICITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞周期蛋白依赖性激酶(CDK)是细胞周期的关键调节因子，在许多癌症中表达上调。CKS是一种已知能与磷酸盐结合的CDK亚基。我们正在探索CKS作为底物靶向亚基，将CDK带到已经被磷酸化启动的底物上。这些结晶学研究的具体目标是解决与磷酸肽结合的CKS的结构，以了解底物专一性的分子基础。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cyclin dependent kinases (Cdk) are critical regulators of the cell cycle and are upregulated in many cancers. Cks is a Cdk subunit that is known to bind phosphate. We are exploring Cks as a substrate targeting subunit that brings Cdk to substrates already primed by phosphorylation. The specific goal of these crystallographic studies is to solve the structure of Cks bound to phosphopeptides to understand the molecular basis for substrate specificity.