INCISIVE OF NUCLEIC ACID CONFORMATIONAL HETEROGENEITY: DNA BULGES
INCISIVE OF NUCLEIC ACID CONFORMATIONAL HETEROGENEITY: DNA BULGES
批准号:
8170218
负责人:
Xuan-Zheng Peter Shi
金额:
$0.1万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-05-01 至 2011-02-28
关键词:
Computer Retrieval of Information on Scientific Projects DatabaseDNAFundingGoldGrantHeterogeneityInstitutionLabelLeftLengthMeasuresNucleic AcidsNucleotidesProbabilityResearchResearch PersonnelResourcesSeriesSolutionsSourceTechniquesUnited States National Institutes of Healthbaseflexibilitynanocrystalresearch study
中文摘要
点击翻译按钮获取中文摘要
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Using small-angle x-ray scattering interference between gold nanocrystal labels, Mathew-Fenn et al. have measured the end-to-end length for a series of DNA double helices in solution. This newly developed Au-SAXS-based x-ray ruler provides the distance probability distribution of an ensemble of molecules. Thus, this technique provides a unique and powerful probe of macromolecular conformational heterogeneity. To establish this ability of this x-ray ruler technique, we propose to study the conformational flexibility and heterogeneity in DNA helices with bulged nucleotides. A-bulges have been shown to bend DNA, giving a good starting point for these studies, but the degree of underlying conformational heterogeneity is unknown. We will compare the effect of A and U bulges of length 1-5 on DNA bending and heterogeneity. We are applying for rapid access because Dr. Mathew-Fenn, the originator of this technique, will leave Stanford in mid-April and we would like to carry out initial SAXS experiments before her departure. Mathew-Fenn et al. have measured the end-to-end length for a series of DNA double helices in solution. This newly developed Au-SAXS-based x-ray ruler provides the distance probability distribution of an ensemble of molecules. Thus, this technique provides a unique and powerful probe of macromolecular conformational heterogeneity. To establish this ability of this x-ray ruler technique, we propose to study the conformational flexibility and heterogeneity in DNA helices with bulged nucleotides. A-bulges have been shown to bend DNA, giving a good starting point for these studies, but the degree of underlying conformational heterogeneity is unknown. We will compare the effect of A and U bulges of length 1-5 on DNA bending and heterogeneity.
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