STRUCTURAL INVESTIGATIONS ON THE ETOH BMC OF E COLI, ENGINEERED ALLOSTERIC PROT

大肠杆菌 ETOH BMC 的结构研究，工程变构保护

• 批准号: 8170290
• 负责人: Martin Sagermann
• 金额: $ 0.13万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170290
• 关键词: Cholesterol Oxidase; Chromobacterium; Computer Retrieval of Information on Scientific Projects Database; Engineering; Escherichia coli; Ethanolamine Ammonia-Lyase; Ethanolamines; Funding; Grant; Institution; Investigation; Molecular; Proteins; Research; Research Personnel; Resources; Solvents; Source; Structure; Time; United States National Institutes of Health; Work; beamline

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Beam time is requested for three ongoing projects regarding 1) the structure and function of the bacterial ethanolamine ammonia lyase microcompartment of, E. coli 2) the engineering of allosteric functions into proteins and 3) the structure and function of the solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1. The preliminary work on all research directions has resulted in crystals or crystalline material that requires high-energy x-ray sources. In all cases, we request access to monochromatic beamlines as most crystallographic structures are likely to be solved by molecular replacement. With regards to the work on the bacterial ethanolamine microcompartment, current work on the EutQ protein (see below) may require access to MAD beamlines.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前正在进行的三个研究项目需要束流时间：1）大肠杆菌乙醇胺氨裂解酶微区室的结构和功能; 2）将变构功能改造成蛋白质和3）来自色杆菌属DS-1的溶剂稳定胆固醇氧化酶的结构和功能。所有研究方向的初步工作都产生了需要高能X射线源的晶体或晶体材料。在所有情况下，我们要求使用单色光束线，因为大多数晶体结构可能通过分子置换来解决。关于细菌乙醇胺微区室的工作，目前对EutQ蛋白的工作（见下文）可能需要使用MAD光束线。