STRUCTURAL INVESTIGATIONS ON THE ETOH BMC OF E COLI, ENGINEERED ALLOSTERIC PROT

大肠杆菌 ETOH BMC 的结构研究，工程变构保护

• 批准号: 8362289
• 负责人: Martin Sagermann
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362289
• 关键词: Cholesterol Oxidase; Chromobacterium; Engineering; Escherichia coli; Ethanol; Ethanolamine Ammonia-Lyase; Ethanolamines; Funding; Grant; Investigation; Molecular; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resources; Solvents; Source; Structure; Time; United States National Institutes of Health; Work; beamline; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Beam time is requested for three ongoing projects regarding 1) the structure and function of the bacterial ethanolamine ammonia lyase microcompartment of, E. coli 2) the engineering of allosteric functions into proteins and 3) the structure and function of the solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1. The preliminary work on all research directions has resulted in crystals or crystalline material that requires high-energy x-ray sources. In all cases, we request access to monochromatic beamlines as most crystallographic structures are likely to be solved by molecular replacement. With regards to the work on the bacterial ethanolamine microcompartment, current work on the EutQ protein (see below) may require access to MAD beamlines.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 三个正在进行的项目需要BEAM时间，这些项目涉及1)细菌乙醇胺氨解酶微室的结构和功能，2)将变构功能改造成蛋白质和3)来自铬杆菌的溶剂稳定的胆固醇氧化酶的结构和功能。DS-1。所有研究方向的初步工作都产生了需要高能X射线源的晶体或晶体材料。在所有情况下，我们都要求使用单色光束线，因为大多数晶体结构很可能通过分子替换来解决。关于细菌乙醇胺微隔室的工作，目前关于EutQ蛋白的工作(见下文)可能需要进入MAD光束线。