STRUCTURAL STUDIES OF THE ACTIN ORGANIZERS SPIRE AND FMN2

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Spire和Cappuccino是果蝇卵母细胞极化的两种肌动蛋白组织者。对它们的哺乳动物直系同源物的研究表明，这种信号联系存在于人类Spir 1和Fmn 2之间。为了更好地理解这些蛋白质如何在分子水平上相互作用，我们单独结晶了约25 kDa的人Spir 1区域，或与对应于其结合元件Fmn 2的肽复合。将通过NE-CAT在APS 24 ID-E获得高分辨率衍射数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Spire and Cappuccino are two actin organizers that collaborate to induce oocyte polarization in Drosophila. Studies with their mammalian orthologues have demonstrated this signaling link exists between human Spir1 and Fmn2. To better understand how these proteins interact at a molecular level, we crystallized a ~25 kDa region of human Spir1 alone, or in complex with a peptide corresponding to its binding element of Fmn2. High resolution diffraction data will be obtained at APS 24ID-E via NE-CAT.