X-RAY CRYSTALLOGRAPHIC STUDIES OF RIBOSOMAL S6 KINASE 2 (RSK2)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 90 kDa核糖体S6激酶2(RSK2)是一种重要的丝氨酸-苏氨酸激酶，广泛表达于多种生长因子中。RSK通路是癌细胞增殖的关键调控因子。在人类中，RSK2基因突变表现为以严重的精神运动迟缓为特征的Coffin-Lowry综合征。阐明RSK2全长的分子结构是理解其激活机制的关键。RSK2属于一个特殊的丝氨酸-苏氨酸激酶家族，它包含两个不同的激酶结构域，通过连接区连接在一起。丝裂原和应激调节蛋白1(MSK1)与RSK2同源，具有相同的结构域结构。RSK2和MSK1这两种蛋白质的晶体结构都不可用。我们计划测定全长RSK2和MSK1的晶体结构。为了得到已经纯化的Se/Met全长MSK1的数据，我们需要在Se/Met蛋白的Se边缘能量处进行反常衍射实验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The 90-kDa ribosomal S6 kinase 2 (RSK2) is important serine-threonine kinase broadly expressed in response to various growth factors. RSK pathway is a key regulator of cancer cell proliferation. In humans, RSK2 gene mutations are manifested in Coffin-Lowry syndrome characterized by severe psychomotor retardation. Elucidating the molecular structure of full length RSK2 is essential for understanding its activation mechanism. RSK2 belongs to the family of unusual serine-threonine kinases that contain two distinct kinase domains connected by a linker region. The mitogen- and stress-reguated kinase 1 (MSK1) is close homologue to RSK2 with the same domain organization. The crystal structure of both proteins, RSK2 and MSK1 are not available. We plan to determine crystals structure of full length RSK2 and MSK1. We will need to perform anomalous diffraction experiments at Se-edge energy for Se/Met protein in order to get the data for Se/Met full length MSK1, which is already purified.