HARDWARE FOR CHALLENGING SAMPLES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 第三代同步加速器大分子结晶光束线的趋势是使用日益复杂的物体(例如，多酶复合体、核糖体等生物分子机器)的逐渐变小(&lt；50微米)的晶体。这两种趋势都依赖于极稳定、高亮度的X射线光学技术、高速数据采集技术以及弱衍射样衍射测量中的背景(噪声)最小化技术的发展。此外，由于任何特定用户对我们的设施的使用都非常有限，我们迫切需要最大限度地提高我们的光束线的效率。核心2试图开发特定的技术和方法来回答这些问题。 我们在核心2目标方面的努力侧重于以下几个方面： 1)继续发展我们的光束稳定方法。 2)改进了我们探测器配置系统的灵活性和校准。 3)在24-ID-C上安装样例机器人装载机(在SPID 0099中讨论)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The trend at third generation synchrotron macromolecular crystallographic beam lines is towards use of progressively smaller (<50 micron) crystals of objects of increasing complexity (e.g. multienzyme complexes, biomolecular machines like the ribosome). Both trends depend upon development of technologies for extremely stable, high brilliance x-ray optics, high speed data collection, and minimization background (noise) in diffraction measurements from weakly diffracting samples. Additionally, since any given user has very limited access to our facilities, we have a strong imperative for maximizing the efficiency of our beam lines. Core 2 attempts to develop specific technologies and methods to answer these problems. Efforts in our Core 2 goals have focused on the following: 1) Continued development of our beam stabilization methodologies. 2) Improvements in the flexibility and calibration of our detector configuration systems. 3) Installation of a sample robotic loader on 24-ID-C (discussed in SPID 0099).