HARDWARE FOR CHALLENGING SAMPLES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 第三代同步加速器大分子晶体光束线的趋势是使用越来越小（<50微米）的晶体，这些晶体的对象越来越复杂（例如多酶复合物，生物分子机器，如核糖体）。 这两种趋势都依赖于技术的发展，以实现极其稳定、高亮度的X射线光学、高速数据收集和最小化弱衍射样品衍射测量中的背景（噪声）。 此外，由于任何特定用户对我们设施的访问都非常有限，因此我们必须最大限度地提高光束线的效率。 核心2试图开发特定的技术和方法来回答这些问题。 我们在核心2目标方面的努力集中在以下方面： 第一章 持续发展我们的光束稳定方法。 （二） 提高我们探测器配置系统的灵活性和校准。 第三章 在24-ID-C上安装样品机器人装载机（在SPID 0099中讨论）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The trend at third generation synchrotron macromolecular crystallographic beam lines is towards use of progressively smaller (<50 micron) crystals of objects of increasing complexity (e.g. multienzyme complexes, biomolecular machines like the ribosome). Both trends depend upon development of technologies for extremely stable, high brilliance x-ray optics, high speed data collection, and minimization background (noise) in diffraction measurements from weakly diffracting samples. Additionally, since any given user has very limited access to our facilities, we have a strong imperative for maximizing the efficiency of our beam lines. Core 2 attempts to develop specific technologies and methods to answer these problems. Efforts in our Core 2 goals have focused on the following: 1) Continued development of our beam stabilization methodologies. 2) Improvements in the flexibility and calibration of our detector configuration systems. 3) Installation of a sample robotic loader on 24-ID-C (discussed in SPID 0099).