CRYSTALLOGRAPHY OF PROLINE CATABOLIC ENZYMES, PHOSPHATASES, AND PARVALBUMINS
脯氨酸分解酶、磷酸酶和小清蛋白的晶体学
基本信息
- 批准号:8169278
- 负责人:
- 金额:$ 0.7万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-04-01 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:Acid PhosphataseAffinityApoptosisBacteriaBindingBirdsBuffersCalciumCalcium-Binding ProteinsCatabolismComplexComputer Retrieval of Information on Scientific Projects DatabaseCrystallographyDataDiseaseEF Hand MotifsElectronsEnzymesFundingGenerationsGlutamatesGoalsGrantHistidineInborn Genetic DiseasesInstitutionLengthMalignant NeoplasmsMetal Ion BindingMetalsOxidation-ReductionOxidoreductaseParvalbuminsPhosphoric Monoester HydrolasesPlayProlineProline DehydrogenaseRattusResearchResearch PersonnelResolutionResourcesRoleSourceStructureSubstrate SpecificitySuperoxidesSystemUnited States National Institutes of HealthWaterX ray diffraction analysisX-Ray Diffractionanalogavian thymic hormonebasecarboxylateoxidationphosphomonoesterprotein functionpyrroline
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The goal of this project is determine crystal structures of proline catabolic enzymes, phosphatases, and parvalbumins. The proline catabolic enzymes proline dehydrogenase (PRODH) and pyrroline-5-carboxylate dehydrogenase (P5CDH) catalyze the 4-electron oxidation of proline to glutamate. These enzymes play important roles in cellular redox control, superoxide generation, apoptosis, and cancer. Inborn errors of proline catabolism result in hyperprolinemia disorders Type I (PRODH) and Type II (P5CDH). In some bacteria, the two enzymes are fused into the bifunctional enzyme, proline utilization A (PutA). PutAs thus serve as systems for studying substrate channeling. The goal of part of the project is to collect high resolution single crystal X-ray diffraction data for several proline catabolic enzymes, including monofunctional PRODH and P5CDH enzymes, PutA domains, and full-length PutAs. Phosphatases catalyze the transfer of a phosphoryl group from phosphomonoesters to water. Phosphatases under study include histidine acid phosphatases (HAPs) and class C acid phosphatases (CCAPs). Our main goal is to determine crystal structures of these phosphatases complexed with substrates and substrate analogues to elucidate the basis for substrate specificity. Parvalbumins (PVs) are small EF-hand calcium-binding proteins that function mainly in calcium buffering. The goal of this part of the project is to elucidate the structural basis for the different metal ion-binding signatures of three representative parvalbumins that span a wide range of metal binding affinity: avian thymic hormone (ATH) and rat beta-PV and avian parvalbumin 3.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JOHN J TANNER其他文献
JOHN J TANNER的其他文献
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{{ truncateString('JOHN J TANNER', 18)}}的其他基金
STRUCTURAL STUDIES OF PHOSPHATASES, AND PARVALBUMINS
磷酸酶和小清蛋白的结构研究
- 批准号:
8361652 - 财政年份:2011
- 资助金额:
$ 0.7万 - 项目类别:
Structural Studies of the Multifunctional PutA Protein
多功能PutA蛋白的结构研究
- 批准号:
6465985 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Structural Studies of the Multifunctional PutA Protein
多功能PutA蛋白的结构研究
- 批准号:
7009382 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Coordination of functions by proline metabolic proteins.
脯氨酸代谢蛋白的功能协调。
- 批准号:
9115668 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Coordination of functions by proline metabolic proteins.
脯氨酸代谢蛋白的功能协调。
- 批准号:
9264540 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Coordination of functions by proline metabolic proteins
脯氨酸代谢蛋白的功能协调
- 批准号:
7527359 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Structural Studies of the Multifunctional PutA Protein
多功能PutA蛋白的结构研究
- 批准号:
6888299 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Coordination of functions by proline metabolic proteins
脯氨酸代谢蛋白的功能协调
- 批准号:
8062111 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
Coordination of functions by proline metabolic proteins
脯氨酸代谢蛋白的功能协调
- 批准号:
7653787 - 财政年份:2002
- 资助金额:
$ 0.7万 - 项目类别:
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