STRUCTURAL STUDIES OF BACTERIAL RNA-BASED PHAGE RESPONSE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 到目前为止，我们已经克隆，表达和表征了不少于20个CRISPR（重复的规则间隔短回文重复序列）相关蛋白的成员。 该家族的成员涉及RNA干扰的细菌类似物，其中外源遗传物质的片段被掺入宿主的基因组中，然后用于同源DNA/RNA的靶向破坏。 我们的主要目标是确定CRISPR和相关途径中涉及的每种蛋白质的确切作用。 作为我们研究的一部分，我们对似乎适合晶体学的蛋白质进行结构研究。 到目前为止，我们已经结晶了三个这样的蛋白质，并完成了一个高分辨率的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. So far, we have cloned, expressed, and characterized no less than 20 members of the CRISPR ( Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins. Members of this family are implicated in a bacterial analog of RNA interference whereby segments of genetic material of foreign origin are incorporated into a host's genome and are then later used for targeted destruction of homologous DNA/RNA. Our primary goal is to determine the exact role of each protein implicated in the CRISPR and associated pathways. As part of our investigations, we undertake structural studies on proteins that appear amenable to crystallography. To date, we have crystallized three such proteins and have completed one high resolution structure.