STRUCTURAL STUDIES OF BACTERIAL RNA-BASED PHAGE RESPONSE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 到目前为止，我们已经克隆、表达和鉴定了至少20个CRISPR(簇状规则间隔短回文重复)相关蛋白。这个家族的成员涉及一种细菌模拟的RNA干扰，即外来遗传物质的片段被合并到宿主的基因组中，然后被用于有针对性地破坏同源DNA/RNA。我们的主要目标是确定CRISPR和相关通路中涉及的每种蛋白质的确切作用。作为我们研究的一部分，我们对看似符合结晶学的蛋白质进行结构研究。到目前为止，我们已经结晶了三个这样的蛋白质，并完成了一个高分辨结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. So far, we have cloned, expressed, and characterized no less than 20 members of the CRISPR ( Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins. Members of this family are implicated in a bacterial analog of RNA interference whereby segments of genetic material of foreign origin are incorporated into a host's genome and are then later used for targeted destruction of homologous DNA/RNA. Our primary goal is to determine the exact role of each protein implicated in the CRISPR and associated pathways. As part of our investigations, we undertake structural studies on proteins that appear amenable to crystallography. To date, we have crystallized three such proteins and have completed one high resolution structure.