ANAPHASE PROMOTING COMPLEX E3 UBIQUITIN LIGASE ACTIVITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 后期促进复合体(APC)是一种多蛋白E3泛素连接酶，通过促进泛素介导的关键细胞周期调节蛋白的蛋白分解来控制细胞分裂。在酿酒酵母和庞贝葡萄球菌的细胞分裂周期(CDC)突变体的经典筛选中，APC亚基的许多基因被鉴定出来，这突显了APC亚单位折叠和组装的重要性。~13个APC亚基可以广泛地划分为亚复合体，其中一个亚基包含一个cullin亚基和一个环亚单位，这使得APC成为E3泛素连接酶家族中最大的成员-cullin-环连接酶。另一个亚复合体包含小的热休克蛋白CDC26和多种含有四肽(TPR)基序的蛋白。其他APC亚复合体含有较少的基序来指示其结构和/或功能。我们正在研究APC的结构和功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Anaphase Promoting Complex (APC) is a multiprotein E3 ubiquitin ligase that controls cell division by promoting ubiquitin-mediated proteolysis of key cell cycle regulatory proteins. The importance of the folding and assembly of APC subunits is underscored by the fact that many of their genes were identified in classic screens for cell division cycle (Cdc) mutants in S. cerevisiae and S. pombe. The ~13 APC subunits can be broadly divided into subcomplexes, one of which contains a cullin subunit and a RING subunit, making the APC a distal member of the largest family of E3 ubiquitin ligases, the Cullin-RING ligases. Another subcomplex contains the small heat shock protein CDC26 and multiple proteins containing tetratricopeptide (TPR) motifs. Other APC subcomplexes contain fewer motifs to indicate their structure and/or function. We are studying the structure and function of the APC.