ANAPHASE PROMOTING COMPLEX E3 UBIQUITIN LIGASE ACTIVITY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 后期促进复合物（APC）是一种多蛋白E3泛素连接酶，通过促进泛素介导的关键细胞周期调控蛋白的蛋白水解来控制细胞分裂。 APC亚基的折叠和组装的重要性被以下事实所强调，即它们的许多基因在S. cerevisiae和S.粟球。 约13个APC亚基可大致分为亚复合物，其中一个亚复合物含有Cullin亚基和RING亚基，使APC成为E3泛素连接酶最大家族Cullin-RING连接酶的远端成员。 另一个亚复合物含有小的热休克蛋白CDC 26和多种含有三肽（TPR）基序的蛋白质。 其他APC亚复合物含有较少的基序来指示它们的结构和/或功能。 我们正在研究APC的结构和功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Anaphase Promoting Complex (APC) is a multiprotein E3 ubiquitin ligase that controls cell division by promoting ubiquitin-mediated proteolysis of key cell cycle regulatory proteins. The importance of the folding and assembly of APC subunits is underscored by the fact that many of their genes were identified in classic screens for cell division cycle (Cdc) mutants in S. cerevisiae and S. pombe. The ~13 APC subunits can be broadly divided into subcomplexes, one of which contains a cullin subunit and a RING subunit, making the APC a distal member of the largest family of E3 ubiquitin ligases, the Cullin-RING ligases. Another subcomplex contains the small heat shock protein CDC26 and multiple proteins containing tetratricopeptide (TPR) motifs. Other APC subcomplexes contain fewer motifs to indicate their structure and/or function. We are studying the structure and function of the APC.