GLOBAL ANALYSIS OF HISTONE SUBTYPE COMPOSITION IN C ELEGANS SPERM USING MUDPIT

使用 MUDPIT 对线虫精子中组蛋白亚型组成进行全局分析

• 批准号: 8171404
• 负责人: Diana S. Chu
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171404
• 关键词: Architecture; Caenorhabditis elegans; Cells; Chromatin; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Development; Embryo; Embryonic Development; Epigenetic Process; Funding; Genome; Grant; Histocompatibility Testing; Histones; Institution; Mass Spectrum Analysis; Nature; Nucleosomes; Oocytes; Organism; Orthologous Gene; Phase; Post-Translational Protein Processing; Protamines; Proteins; Research; Research Personnel; Resources; Source; Technology; Undifferentiated; United States National Institutes of Health; Variant; cell type; chromatin protein; embryonic protein; novel strategies; sperm analysis; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sperm are remarkable cells specialized for the delivery of the paternal genome to the oocyte, while embryos consist of rapidly dividing, largely undifferentiated cells. Unlike embryos, sperm have jettisoned most cellular components, have highly compacted chromatin, and are thought to be largely transcriptionally inactive. Thus, sperm rely on substitution histones and post-translational modification (PTM) of existing proteins for development and function. In other organisms, replacement of S-phase histones by histone variants and protamines fundamentally changes chromosome architecture at the nucleosome level. How chromatin differs between these two tissue types has been largely unexplored. To investigate the nature of C. elegans sperm chromatin composition, we conducted a global analysis of sperm and embryo chromatin proteins using Multidimensional Protein Identification Technology (MudPIT) mass spectroscopy. Our novel approach to determine chromatin composition between disparate cell types has identified over 1000 proteins from embryos and sperm each, laying the groundwork for analysis of other histone subtypes as well as protamine orthologs. In addition, our ongoing large-scale identification of histone PTMs is a valuable resource to examine how paternal epigenetic marks contribute to embryonic development.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 精子是专门用于将父亲基因组递送到卵母细胞的显着细胞，而胚胎则由迅速分裂的，很大程度上未分化的细胞组成。与胚胎不同，精子已经抛弃了大多数细胞成分，具有高度压实的染色质，被认为在很大程度上是不活跃的。因此，精子依赖于现有蛋白质的替代组蛋白和翻译后修饰（PTM）进行开发和功能。在其他生物体中，由组蛋白变体和精蛋白替代S期组蛋白，从根本上改变了核小体水平的染色体结构。这两种组织类型之间的染色质如何在很大程度上没有探索。 为了研究秀丽隐杆线虫精子染色质组成的性质，我们使用多维蛋白鉴定技术（MUDPIT）质谱进行了全球精子和胚胎染色质蛋白的分析。 我们确定不同细胞类型之间染色质组成的新方法已从胚胎和每个精子中鉴定出了1000多种蛋白质，为分析其他组蛋白亚型以及精神素直系同源物奠定了基础。此外，我们正在进行的大规模识别组蛋白PTM是一个宝贵的资源，用于研究父亲表观遗传标记如何有助于胚胎发育。