IDENTIFICATION OF NOVEL HUMAN SLX1 AND SLX4 PARTNERS

新型人类 SLX1 和 SLX4 伙伴的鉴定

• 批准号: 8171307
• 负责人: PIERRE-HENRI GAILLARD
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171307
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Repair; Docking; Event; Exposure to; Flap Endonucleases; Funding; Genetic Recombination; Grant; Holliday Junction Resolvases; Human; Institution; Proteins; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; crosslink; endonuclease; homologous recombination; novel; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have recently identified and functionally characterized the human SLX1 and SLX4 proteins. Both proteins appear to be required in response to MMS treatment while only SLX4 is required for survival following exposure to DNA inter-strand crosslinking agents. SLX1 is a 5' flap endonuclease which is strongly stimulated by its association with SLX4. Furthermore, the SLX1-SLX4 complex displays a robust Holliday junction resolvase activity suggesting that it is involved in homologous recombination events. Importantly, we have shown that SLX4 also interacts with the XPF and MUS81 subunits of the XPF-ERCC1 and MUS81-EME1 DNA repair/recombination endonucleases and have proposed that SLX4 may act as a docking platform for multiple structure-specific endonucleases.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们最近已经确定和功能特性的人SLX 1和SLX 4蛋白。这两种蛋白质似乎都是响应MMS处理所需的，而在暴露于DNA链间交联剂后存活仅需要SLX 4。SLX 1是一种5'侧翼核酸内切酶，其通过与SLX 4的结合而被强烈刺激。此外，SLX 1-SLX 4复合物显示出稳健的霍利迪连接解离酶活性，表明其参与同源重组事件。重要的是，我们已经证明SLX 4还与XPF-ERCC 1和MUS 81-EME 1 DNA修复/重组核酸内切酶的XPF和MUS 81亚基相互作用，并提出SLX 4可能作为多个结构特异性核酸内切酶的对接平台。