ROLES FOR CASEIN KINASE 2 IN KINETOCHORE REGULATION

酪蛋白激酶 2 在动粒调节中的作用

• 批准号: 8171239
• 负责人: Yuandong Peng
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171239
• 关键词: CCL14 gene; Catalytic Domain; Cell division; Cell physiology; Centromere; Chromosome Segregation; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Ensure; Eukaryota; Event; Funding; Grant; Homologous Gene; Human; Institution; Kinetochores; Link; Microtubules; Mitotic spindle; Monitor; Phosphotransferases; Proteins; Regulation; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; United States National Institutes of Health; Yeasts; casein kinase II; centromere protein C; genome-wide; protein complex; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The kinetochore is a multi-protein complex that assembles on the centromere and attaches chromosomes to spindle microtubules during cell division. Kinetochore attachments are tightly monitored to ensure faithful chromosome segregation. A recent genome-wide yeast two-hybrid screen focusing on the mitotic spindle identified interactions between the essential kinetochore protein Mif2 (S. cerevisiae homolog of human CENP-C) and two Casein Kinase 2 (CK2) subunits, Cka2 and Ckb2. CK2, a ubiquitous and highly conserved kinase in eukaryotes, is composed of two catalytic subunits (Cka1 and Cka2) and two regulatory subunits (Ckb1 and Ckb2). Although CK2 has been linked to a variety of cellular processes, how CK2 regulates these diverse events is largely unclear. In this study, we investigated the roles of CK2 in mitotic spindle function.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 动粒是一种多蛋白复合体，在细胞分裂过程中，它聚集在着丝粒上并将染色体附着在纺锤体微管上。密切监测动粒附着以确保忠实的染色体分离。最近的一个全基因组酵母双杂交筛选集中在有丝分裂纺锤体确定之间的相互作用的基本动粒蛋白Mif 2（S。人CENP-C的酿酒酵母同源物）和两个酪蛋白激酶2（CK 2）亚基Cka 2和Ckb 2。CK 2是真核生物中普遍存在的高度保守的激酶，由两个催化亚基（Cka 1和Cka 2）和两个调节亚基（Ckb 1和Ckb 2）组成。虽然CK 2与多种细胞过程有关，但CK 2如何调节这些不同的事件在很大程度上尚不清楚。在这项研究中，我们研究了CK 2在有丝分裂纺锤体功能中的作用。