CYSTEINE DIOXYGENASE

半胱氨酸双加氧酶

• 批准号: 8170677
• 负责人: Quan Hao
• 金额: $ 0.78万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170677
• 关键词: Alzheimer' s Disease; Amino Acid Sequence; Carbon; Catabolism; Cell Respiration; Chronic; Clinical Data; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Cysteine; Cysteine dioxygenase; Ensure; Funding; Genetic Polymorphism; Gluconeogenesis; Grant; Homologous Protein; Human; Institution; Iron; Maintenance; Molecular; Motor Neuron Disease; Oxygen; Play; Protein Databases; Proteins; Reaction; Research; Research Personnel; Resources; Rheumatoid Arthritis; Role; Severities; Source; Structure; Substrate Specificity; Sulfhydryl Compounds; Sulfinic Acids; Sulfur; Taurine; United States National Institutes of Health; cytotoxic; metalloenzyme; nervous system disorder; prevent; sulfation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cysteine dioxygenase (CDO, EC 1.13.11.20) is an Fe2+ metalloenzyme that adds molecular oxygen to the thiol group of cysteine to form the sulfinic acid called cysteinesulfinate. By catalyzing the first step in the oxidative metabolism of cysteine, CDO plays a key role in cysteine catabolism, in provision of cysteine carbon for gluconeogenesis or oxidative metabolism, in taurine synthesis, and in supply of inorganic sulfur for sulfation reactions. Perhaps more importantly, CDO is responsible for ensuring the maintenance of low cellular cysteine concentrations and preventing its cytotoxic and excitotoxic effects. Human clinical data strongly suggests the existence of genetic polymorphisms in CDO, with low expression of functional CDO being associated with the occurrence, severity, or rate of progression of rheumatoid arthritis as well as several chronic neurological disorders (e.g., motor neuron disease, Alzheimers disease).Very little is known about the structure or catalytic mechanism of CDO other than its amino acid sequence, substrate specificity, and its single iron atom that is required for catalytic activity. Searches (BLASTP) of existing databases for proteins with similar domain structures yield a number of CDOs or putative gene products that are homologous to CDO but no other homologous proteins.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 半胱氨酸双加氧酶（CDO，EC 1.13.11.20）是一种Fe 2+金属酶，其将分子氧添加到半胱氨酸的巯基以形成称为半胱氨酸亚磺酸盐的亚磺酸。 通过催化半胱氨酸氧化代谢的第一步，CDO在半胱氨酸催化剂中起关键作用，在提供半胱氨酸碳用于代谢或氧化代谢中，在牛磺酸合成中，以及在提供无机硫用于硫酸化反应中。 也许更重要的是，CDO负责确保维持低细胞半胱氨酸浓度并防止其细胞毒性和兴奋性毒性作用。 人类临床数据强烈表明CDO中存在遗传多态性，功能性CDO的低表达与类风湿性关节炎以及几种慢性神经系统疾病（例如，运动神经元疾病、阿尔茨海默病）。除了CDO的氨基酸序列、底物特异性以及催化活性所需的单个铁原子之外，人们对CDO的结构或催化机制知之甚少。对具有相似结构域结构的蛋白质的现有数据库的搜索（BLASTP）产生了许多CDO或推定的基因产物，其与CDO同源，但没有其他同源蛋白质。