STRUCTURAL STUDIES OF HIV-1 REVERSE TRANSCRIPTASE (RT)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们将制备HIV-1逆转录酶（RT）的大分子复合物，RT是HIV-1病毒生命周期中的重要组成部分，具有核酸和核苷及非核苷逆转录酶抑制剂。 这些配合物的三维结构将由X射线晶体学方法确定。通过这些研究，我们希望能够详细了解RT功能抑制的基本原理以及对这些抑制剂的耐药性的出现。 最终，我们希望将从我们的结构研究中获得的信息应用于以更好的抗HIV药物的形式促进新的有效的治疗策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will prepare macromolecular complexes of HIV-1 reversetranscriptase (RT), an essential component in the life cycle of theHIV-1 virus, with nucleic acid and nucleoside and nonnucleoside reversetranscriptase inhibitors. The three dimensional structures of thesecomplexes will be determined by x-ray crystallographic methods. Throughthese studies we hope to gain a detailed understanding of thefundamental principles underlying inhibition of RT's function and theemergence of resistance to these inhibitors. Ultimately we hope toapply information garnered from our stuctural studies towards theadvancement of new and effective treatment stategies in the form ofbetter anti-HIV drugs.