STRUCTURAL STUDIES OF HIV-1 REVERSE TRANSCRIPTASE (RT)

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们将制备HIV-1逆转录酶（RT）的大分子复合物，RT是HIV-1病毒生命周期中的重要组成部分，具有核酸和核苷及非核苷逆转录酶抑制剂。 这些配合物的三维结构将通过X射线晶体学方法确定。通过这些研究，我们希望能够详细了解RT功能抑制的基本原理以及对这些抑制剂产生耐药性的原因。 最终，我们希望将从我们的结构研究中获得的信息应用于以更好的抗艾滋病毒药物的形式推进新的有效治疗策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will prepare macromolecular complexes of HIV-1 reverse transcriptase (RT), an essential component in the life cycle of the HIV-1 virus, with nucleic acid and nucleoside and nonnucleoside reverse transcriptase inhibitors. The three dimensional structures of these complexes will be determined by x-ray crystallographic methods. Through these studies we hope to gain a detailed understanding of the fundamental principles underlying inhibition of RT's function and the emergence of resistance to these inhibitors. Ultimately we hope to apply information garnered from our stuctural studies towards the advancement of new and effective treatment stategies in the form of better anti-HIV drugs.