STRUCTURAL STUDIES OF HIV-1 REVERSE TRANSCRIPTASE (RT)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们将制备HIV-1逆转录酶(RT)与核酸、核苷和非核苷逆转录酶抑制剂的大分子复合体。RT是HIV-1病毒生命周期中的重要组成部分。这些配合物的三维结构将由X射线结晶学方法确定。通过这些研究，我们希望详细了解抑制RT功能的基本原理以及对这些抑制剂的耐药性的出现。最终，我们希望将从我们的结构研究中获得的信息应用于以更好的抗艾滋病毒药物的形式推进新的有效治疗策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will prepare macromolecular complexes of HIV-1 reverse transcriptase (RT), an essential component in the life cycle of the HIV-1 virus, with nucleic acid and nucleoside and nonnucleoside reverse transcriptase inhibitors. The three dimensional structures of these complexes will be determined by x-ray crystallographic methods. Through these studies we hope to gain a detailed understanding of the fundamental principles underlying inhibition of RT's function and the emergence of resistance to these inhibitors. Ultimately we hope to apply information garnered from our stuctural studies towards the advancement of new and effective treatment stategies in the form of better anti-HIV drugs.