2'-SEME-MODIFIED OLIGONUCLEOTIDES

2-SEME 修饰的寡核苷酸

• 批准号: 8170685
• 负责人: Zhen Huang
• 金额: $ 0.9万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170685
• 关键词: Base Sequence; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Hydration status; Institution; Modification; Nucleic Acids; Nucleic acid sequencing; Pattern; Phase; Positioning Attribute; Proteins; Research; Research Personnel; Resources; Selenium; Source; Structure; Time; United States National Institutes of Health; Work; X-Ray Crystallography; macromolecule; nucleic acid structure; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this work, we would like to further prove that selenium derivatization can greatly facilitate the phase determination and help to resolve the structures of macromolecules. Since the selenium strategy have greatly facilitated the structure determination in the field of protein, it can also be used in nucleic acid field, which actually has been proved by our lab. Besides the phase determination,we also found that selenium modification at the 2' position can make the crystals grow much faster in some sequences(less than one hour~!)than native ones. Here is an example, we got the crystals for this selenium contained nucleic acid sequence (5'-GU(2'-Se)GTACAC-3') in a short time. So, in addition to check the phase determination to widen it's application, we also like to know more structural details from the X-ray crystallography, such as hydration pattern and sugar packing, etc. It will be helpful to solve the cryallization and phase problem, the two main difficulties in the field of nucleic acid structure study.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本工作进一步证明硒衍生化可以极大地促进物相测定，并有助于解析大分子的结构。由于硒策略在蛋白质领域的结构测定中起到了很大的作用，因此它也可以应用于核酸领域，这一点在本实验室也得到了证实。除了相的确定，我们还发现在2'位的硒修饰可以使晶体在某些序列中生长得更快（小于1小时~！）而不是本地人。这里有一个例子，我们在很短的时间内得到了这种含硒核酸序列（5 '-GU（2'-Se）GTACAC-3 '）的晶体。因此，除了对相测定进行检验以拓宽其应用范围外，我们还希望从X射线晶体学中了解更多的结构细节，如水化模式和糖堆积等，这将有助于解决核酸结构研究中的结晶和相问题这两个主要难题。