SELENIUM-DERIVATIZED NUCLEIC ACIDS (SENA) FOR PHASING, CRYSTALLIZATION AND X-RAY

用于定相、结晶和 X 射线的硒衍生核酸 (SENA)

• 批准号: 8363352
• 负责人: Zhen Huang
• 金额: $ 0.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363352
• 关键词: Complex; Crystallization; Crystallography; Family; Funding; Grant; Laboratories; Light; National Center for Research Resources; Nucleic Acids; Oligonucleotides; Oxygen; Phase; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Roentgen Rays; Selenium; Solutions; Source; Structure; Synchrotrons; United States National Institutes of Health; base; cost; novel; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. My laboratory pioneered and successfully demonstrated a novel derivatization strategy via replacement of oxygen of nucleic acids with selenium. Our research is based on the central hypothe selenium can be used to stably replace oxygen of nucleic acids atom-specifically without significant perturbation, because selenium and oxygen are in the same elemental family. We have successfully demonstrated that the selenium derivatization of nucleic acids can be useful to solve the phase problem. Moreover, we have found that the selenium derivatization can facilitate oligonucleotide crystallization. Thus, this Se-derivatization strategy has great potential to provide novel solutions to both long-standing problems: phasing and crystallization, especially for nucleic acids and protein-nucleic acid complexes. Therefore, our structural research focuses on developing general strategies for phasing, crystallization, and crystal structure determination of nucleic acids and protein complexes.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我的实验室率先并成功地证明了一种新的衍生化策略，通过用硒取代核酸的氧。我们的研究是基于中心假设，硒可以被用来稳定地取代核酸中的氧原子特异性地没有显着的扰动，因为硒和氧是在同一个元素家庭。我们已经成功地证明核酸的硒衍生化可以用于解决相位问题。此外，我们发现硒衍生化可以促进寡核苷酸结晶。因此，这种硒衍生化策略具有很大的潜力，提供新的解决方案，这两个长期存在的问题：定相和结晶，特别是核酸和蛋白质-核酸复合物。因此，我们的结构研究重点是开发核酸和蛋白质复合物的定相，结晶和晶体结构测定的一般策略。