POLARITY IN NETWORKS AND PATHWAYS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 网络和通路中的极性由细胞内特定分子的空间组织、亚细胞分布、动态行为、相互作用和功能决定。要了解网络和通路中极性的基础，需要开发新的方法来测量细胞中分子的浓度、扩散特性和相互作用，需要新的方法来操纵细胞中分子的浓度、位置和相互作用，以及需要新的策略来模拟不同亚细胞隔间中网络和通路中的分子相互作用。细胞分析和建模中心(CCAM)的网络和路径极性技术中心将新的显微镜技术与新的物理配方和计算工具相结合，用于进行活体细胞的定量测量，这些新的物理配方和计算工具可以产生空间上逼真的细胞内动力学定量模型。三个核心技术项目，模型、测量和操纵，为阐明活细胞中的时空动力学提供了一个完整的框架。由技术开发核心开发的工具是由一系列驱动生物学项目(DBP)推动的，这些项目涵盖了细胞生物学中的主要复杂问题。这些DBP都围绕着细胞如何控制其分子成分的位置以及如何利用这种极性来优化细胞功能的问题。在该项目下制定的建模方法方面的进展随后被纳入虚拟单元，以便分发给我们的用户群。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polarity in networks and pathways is determined by the spatial organization, subcellular distribution, dynamic behavior, interactions and functions of specific molecules within the cell. Understanding the basis for polarity in networks and pathways will require development of new methods to measure concentrations, diffusion properties and interactions of molecules in cells, new ways to manipulate concentrations, locations and interactions of molecules in cells, and new strategies to model interactions of molecules in networks and pathways in different subcellular compartments. The Technology Center for Polarity in Networks and Pathways at the Center for Cell Analysis and Modeling (CCAM) integrates new microscope technologies for making quantitative in vivo live cell measurements with new physical formulations and computational tools that produce spatially realistic quantitative models of intracellular dynamics. Three core technology projects, Model, Measure and Manipulate, provide an integrated framework for elucidating spatiotemporal dynamics in living cells. Development of tools by the Technology Development Cores is motivated by a series of Driving Biological Projects (DBP) that cover major complex problems in cell biology. These DBPs all revolve around the issue of how the cell controls the locations of its molecular components and how that polarity is used to optimize a cellular function. Advances in modeling approaches formulated under this project are subsequently integrated into the Virtual Cell for dissemination to our user base.