STUDY OF THE DOPAMINE D1 AND D2 RECEPTORS LOCALIZATION IN VIVO

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 多巴胺D1和D2受体的定位是非常有争议的。一些实验室报道，100%的纹状体神经元共表达这两种受体，而另一些实验室报道，只有一小部分神经元共表达这两种受体（5 - 20%）。我们的目标是确定这两种受体是否在成人和胚胎神经元中共同定位。为此，我们在实验室中产生了表达用ECFP标记的多巴胺D1 R和用EYFP标记的多巴胺D2 R的敲入小鼠。这些小鼠将用于在正常条件下和滥用药物治疗后观察内源性受体的定位。此外，我们已经将D1 R-ECFP与D2 R-EYFP小鼠交配，以便能够进行FRET研究，从而告诉我们这两种受体是否可能在体内相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The localization of dopamine D1 and D2 receptors is highly controversial. Some laboratories have reported that 100% of striatal neurons co-express the two receptors while others have reported that only a fraction of them does (from 5 to 20%. Our goal is to determine if both receptors co-localize in adult as well as in embryonal neurons. For this, we have generated in our lab knockin mice that express dopamine D1R tagged with ECFP and dopamine D2R tagged with EYFP. These mice will be used to visualize the localization of endogenous receptors under normal conditions and after treatment with drugs of abuse. Furthermore, we have mated D1R-ECFP with D2R-EYFP mice to be able to perform FRET studies that will tell us if these two receptors might interact in vivo.