STUDY OF THE DOPAMINE D1 AND D2 RECEPTORS LOCALIZATION IN VIVO

多巴胺 D1 和 D2 受体体内定位的研究

• 批准号: 8170982
• 负责人: Emiliana Borrelli
• 金额: $ 0.59万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170982
• 关键词: Adult; Aftercare; Computer Retrieval of Information on Scientific Projects Database; Corpus striatum structure; DRD2 gene; Dopamine; Dopamine D2 Receptor; Fluorescence Resonance Energy Transfer; Funding; Goals; Grant; Institution; Laboratories; Mus; Neurons; Partner in relationship; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; drug of abuse; in vivo; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The localization of dopamine D1 and D2 receptors is highly controversial. Some laboratories have reported that 100% of striatal neurons co-express the two receptors while others have reported that only a fraction of them does (from 5 to 20%. Our goal is to determine if both receptors co-localize in adult as well as in embryonal neurons. For this, we have generated in our lab knockin mice that express dopamine D1R tagged with ECFP and dopamine D2R tagged with EYFP. These mice will be used to visualize the localization of endogenous receptors under normal conditions and after treatment with drugs of abuse. Furthermore, we have mated D1R-ECFP with D2R-EYFP mice to be able to perform FRET studies that will tell us if these two receptors might interact in vivo.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多巴胺D_1和D_2受体的定位存在很大争议。一些实验室报告说，100%的纹状体神经元同时表达这两种受体，而另一些实验室报告说，只有一小部分神经元表达这两种受体(从5%到20%)。我们的目标是确定这两种受体是否在成年神经元和胚胎神经元中共定位。为此，我们在实验室培育了表达ECFP标记的多巴胺D1R和EYFP标记的多巴胺D2R的敲击小鼠。这些小鼠将被用来观察正常情况下和滥用药物治疗后内源性受体的定位。此外，我们已经将D1R-ECFP与D2R-EYFP小鼠配对，以便能够进行FRET研究，告诉我们这两个受体是否在体内可能相互作用。