HIGH PRESSURE COOLING OF MYOSIN MOTORS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经获得了肌球蛋白马达(Myosin VI和Dictyostellium myosin II)的晶体，这些晶体可能提供重要的结构信息，帮助捕获ATPase循环中的中间体，并了解肌球蛋白如何在负荷下工作。我们希望与康奈尔大学的Sol M.Gruner博士和Chae Un Kim博士合作，使用他们新开发的高压冷冻冷却方法来收集数据。在正常低温条件下，肌球蛋白晶体的衍射率可超过2.0？在ID27(ESRF法国)上进行的第一次测试表明，它们可以很好地抵抗压力。我们希望，在低温条件下使用高压将为理解肌球蛋白的功能提供新的见解。 因此，我们在CHESS要求两天的光束时间，以测试高压冷冻对这些晶体的衍射的影响，如果成功，将收集完整的原始数据集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have obtained crystals of myosin motor (myosin VI and Dictyostellium myosin II) that can potentially provide important structural information to help trap intermediates in their ATPase cycle and understand how a myosin can work under load. In collaboration with Dr. Sol M. Gruner and Dr. Chae Un Kim at Cornell, we would like to collect data using their newly developed high-pressure cryo-cryocooling approach. Myosin crystals can diffract beyond 2.0 ¿ resolution under normal cryogenic conditions. First tests performed on ID27 (ESRF France) show that they resist pressure quite well. We hope that using high-pressure under cryocooling condition will give new insights to understand how myosin functions. We are therefore requesting two days of beam time at CHESS to test the effects of high-pressure cryocooling on the diffraction of these crystals, and if successful, to collect full native datasets.