HIGH PRESSURE COOLING OF MYOSIN MOTORS

肌球蛋白马达的高压冷却

• 批准号: 8171525
• 负责人: ANNE HOUDUSSE
• 金额: $ 1.34万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171525
• 关键词: ATP phosphohydrolase; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; France; Funding; Grant; Institution; Motor; Myosin ATPase; Myosin Type II; Research; Research Personnel; Resolution; Resources; Source; Testing; Time; United States National Institutes of Health; Work; cryogenics; insight; myosin VI; pressure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have obtained crystals of myosin motor (myosin VI and Dictyostellium myosin II) that can potentially provide important structural information to help trap intermediates in their ATPase cycle and understand how a myosin can work under load. In collaboration with Dr. Sol M. Gruner and Dr. Chae Un Kim at Cornell, we would like to collect data using their newly developed high-pressure cryo-cryocooling approach. Myosin crystals can diffract beyond 2.0 ¿ resolution under normal cryogenic conditions. First tests performed on ID27 (ESRF France) show that they resist pressure quite well. We hope that using high-pressure under cryocooling condition will give new insights to understand how myosin functions. We are therefore requesting two days of beam time at CHESS to test the effects of high-pressure cryocooling on the diffraction of these crystals, and if successful, to collect full native datasets.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经获得了肌球蛋白马达（肌球蛋白VI和Dictyostellium肌球蛋白II）的晶体，这些晶体可以潜在地提供重要的结构信息，以帮助捕获ATP酶循环中的中间体，并了解肌球蛋白如何在负荷下工作。与Sol M博士合作。Gruner和康奈尔大学的Chae Un Kim博士，我们希望使用他们新开发的高压低温冷却方法收集数据。在正常低温条件下，肌球蛋白晶体的分辨率可超过2.0。在ID 27（ESRF France）上进行的第一次测试表明，它们具有很好的耐压性。我们希望在冷冻条件下使用高压将为了解肌球蛋白的功能提供新的见解。 因此，我们要求在国际象棋上使用两天的光束时间来测试高压低温冷却对这些晶体衍射的影响，如果成功，收集完整的本地数据集。