CONTROL OF ULTIDRUG EFFLUX TRANSPORTER ACTIVITY BY CELL SURFACE REORGANIZATION

通过细胞表面重组控制超级药物外排转运蛋白活性

• 批准号: 8169651
• 负责人: AMRO M HAMDOUN
• 金额: $ 2.39万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169651
• 关键词: Animal Model; Award; Biological; Biological Assay; Cell physiology; Cell surface; Cells; Computer Retrieval of Information on Scientific Projects Database; Embryo; Embryonic Development; Epithelial; Funding; Goals; Grant; Institution; Knowledge; Link; Malignant Neoplasms; Membrane; Phase; Regulation; Research; Research Personnel; Resources; Role; Sea Urchins; Source; Teratogens; Translating; United States National Institutes of Health; assisted reproduction; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The two main goals of this research are first, to understand the cell biological regulation of multidrug efflux transport and second, to probe the roles of these transporters in protection and regulation of embryonic development. This proposal links between two bodies of knowledge, one on the cellular physiology of multidrug efflux transport, primarily examined in the context of cancer and epithelial transport, and another focusing the structural changes in cell surface and membrane organization of embryo development. In this proposal, we will investigate the relationship between cell surface changes in early embryo development and changes in the efflux transporter activity focusing on sea urchins as an easily assayed and manipulated model organism. We will also continue efforts, currently ongoing in the K Phase of this award, to translate findings from the sea urchin to the mouse model, in order to characterize the role of these activity changes in protection of the embryo from potential teratogens encountered during assisted reproduction.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 本研究的两个主要目标是第一，了解多药外排转运的细胞生物学调节，第二，探索这些转运蛋白在保护和调节胚胎发育中的作用。这一建议将两个知识体系联系起来，一个是关于多药外排转运的细胞生理学，主要在癌症和上皮转运的背景下进行研究，另一个关注胚胎发育的细胞表面和膜组织的结构变化。在这个提议中，我们将研究早期胚胎发育中细胞表面变化与外排转运蛋白活性变化之间的关系，重点是海胆作为一种易于测定和操作的模式生物。 我们还将继续努力，目前正在该奖项的K阶段，将海胆的发现转化为小鼠模型，以表征这些活性变化在保护胚胎免受辅助生殖期间遇到的潜在致畸剂的作用。