SIGNALING STATE OLIGOMERIZATION OF THE SLR1694 BLUF DOMAIN DURING PHOTOCYCLE

光循环期间 SLR1694 BLUF 结构域的信号状态寡聚化

• 批准号: 8172013
• 负责人: HYOTCHERL IHEE
• 金额: $ 2.56万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172013
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Environmental Risk Factor; Funding; Gene Expression; Grant; Homo; Institution; Life; Ligands; Light; Molecular; Optics; Organism; Oxygen; Photoreceptors; Photosynthesis; Play; Proteins; Reaction; Research; Research Personnel; Resources; Roentgen Rays; Role; Shapes; Signal Transduction; Signal Transduction Pathway; Solutions; Source; Spectrum Analysis; State Interests; Stimulus; Techniques; Tertiary Protein Structure; Time; United States National Institutes of Health; Work; absorption; dimer; receptor; research study; response; time use; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. All living organisms have an ability to respond to biological stimuli such as light, voltage, oxygen, ligands and environmental factors. When applied, each stimulus is converted into a specific cellular response in several steps contributing to signal-transduction pathway. To understand the working mechanism of signal transduction, it is important to identify the molecular units involved in signaling, for example, stimuli receptors. Among the stimuli, light plays an important role in a wide range of biologically important cellular responses including photosynthesis and gene expression (1). Thus far, six kinds of photoreceptors have been identified (2). Among these photoreceptors, the BLUF domain was discovered recently, thus having been studied to a limited extent. In particular, the Slr1694 BLUF domain exists as a homo dimer in solution, but oligomerizes in the dark to form a decamer. This putative signaling state is optically inactive that its formation dynamics is not readily probed using time-resolved optical spectroscopy, for example, transient absorption (TA) spectroscopy. In contrast, time-resolved X-ray scattering techniques are sensitive to the structural changes regardless of the optical activity of the state of interest. For example, time-resolved small-angle X-ray scattering (TR-SAXS) is sensitive to global structural changes such as size and shape of a protein in solution, while time-resolved wide-angle X-ray scattering (TR-WAXS) provides rich information about tertiary and quaternary structural changes of a protein with global sensitivity. Therefore, time-resolved X-ray scattering techniques are suitable for probing the structural dynamics of the signaling state formation in the BLUF domain proteins. Here, we propose the time-resolved experiments on the Slr1694 BLUF domain proteins using TR-SAXS and TR-WAXS techniques to investigate the mechanism of photochemical reaction leading to the formation of signaling state.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 所有生物体都有能力对生物学刺激做出反应，例如光，电压，氧气，配体和环境因素。应用时，每个刺激都会以几个步骤转化为特定的细胞反应，这些步骤有助于信号转导途径。为了了解信号转导的工作机理，重要的是要确定涉及信号传导的分子单位，例如刺激受体。 在刺激中，光在包括光合作用和基因表达在内的广泛的生物学重要细胞反应中起着重要作用（1）。到目前为止，已经鉴定出六种感光体（2）。在这些光感受器中，最近发现了BLUF结构域，因此已在有限的程度上进行了研究。特别是，SLR1694 BLUF域是作为溶液中的同型二聚体而存在的，但在黑暗中寡聚以形成decamer。这种推定的信号传导状态是光学不活跃的，即使用时间分辨光谱法（例如，瞬时吸收（TA）光谱）不容易探测其形成动力学。相比之下，不论感兴趣状态的光学活性如何，时间分辨X射线散射技术对结构变化敏感。例如，时间分辨的小角度X射线散射（TR-saxs）对溶液中蛋白质的大小和形状等全球结构变化敏感，而时间分解的广角X射线散射（TR-WAXS）则提供了有关蛋白质具有全球敏感性的蛋白质的第三纪和二氢结构变化的丰富信息。因此，时间分辨X射线散射技术适合探测BLUF结构蛋白中信号态形成的结构动力学。在这里，我们使用TR-SAXS和TR-WAXS技术在SLR1694 BLUF域蛋白上提出了时间分辨实验，以研究光化学反应的机理，从而导致信号传导的形成。