SATBILIZATION OF ALPHA4, BETA2, ALPHA7 NACHRS USING COMPUTATIONAL METHODS
SATBILIZATION OF ALPHA4, BETA2, ALPHA7 NACHRS USING COMPUTATIONAL METHODS
批准号:
8171917
负责人:
JEFFRY D. MADURA
金额:
$0.11万
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-08-01 至 2013-07-31
关键词:
AcetylcholineAffectAnestheticsBindingCellsCellular MembraneCholinergic ReceptorsComputer Retrieval of Information on Scientific Projects DatabaseComputing MethodologiesFundingGated Ion ChannelGeneral anesthetic drugsGlycine ReceptorsGoalsGrantInstitutionIon Channel GatingLipidsMembraneMembrane ProteinsMuscleMutationNervous system structureNeuraxisNeuronsNeurotransmittersNicotinic ReceptorsNuclear Magnetic ResonancePlayProcessProtein SubunitsProteinsResearchResearch PersonnelResolutionResourcesRoleSiteSourceStructureTransmembrane DomainUnited States National Institutes of Healthreceptorresponsesimulation
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The central nervous system is responsible for coordinating the various processes of the body. Acetylcholine plays an important role in the central nervous system as a neurotransmitter that elicits a variety of cellular responses. The main activity of the acetylcholine neurotransmitter is the excitation of muscle tissue. Acetylcholine interacts with its target cells by binding to acetylcholine receptors in cellular membranes. Nicotinic acetylcholine receptors (nAChRs) are an example of membrane-bound acetylcholine receptor proteins. These receptors are also putative targets of interaction with anesthetic drugs. Most study of the interaction between receptor proteins and anesthetic drugs is technically challenging due to the difficulty of isolating membrane proteins and limitations when using nuclear magnetic resonance (NMR) to elucidate receptor structure at a high resolution. Within the membrane, both neurotransmitter ion-gated channels and cys-loop regions of membranous proteins have been implicated as sites of interaction with general anesthetics. Studies have thus far demonstrated that nAChRs are composed of various protein subunits which span the membrane and extend extracellularly while the pore of the receptor is located inside the membrane with a surrounding frame that separates it from the lipids within the membrane. Neuronal nAChRs can be composed of different subunits, such as a heteromeric nAChR of two a4 and three b2 subunits, or a homomeric nAChR containing five a7 subunits. Our ultimate goal is to gain an understanding of the mechanism by which anesthetic drugs affect the nervous system. In order to reach this goal, computational methods will be used in an attempt to find mutations of the b2 and a7 neuronal nAChRs and the a1 glycine receptor that are more stable at a lower pH. Computational methods of simulation and structural determination will be used in an effort to stabilize the a4, b2, and a7 proteins of the transmembrane domain of the nAChRs. The a1 glycine receptor will also be studied similarly.
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SATBILIZATION OF ALPHA4, BETA2, ALPHA7 NACHRS USING COMPUTATIONAL METHODS
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批准号:8364301
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项目类别:
-
资助金额:$0.11万
-
财政年份:2011
-
负责人:JEFFRY D. MADURA
-
依托单位:
CRCNS: Computational and experimental study of dopamine and serotonin transporter
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批准号:8274837
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项目类别:
-
资助金额:$28.8万
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财政年份:2009
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负责人:JEFFRY D. MADURA
-
依托单位:
CRCNS: Computational and experimental study of dopamine and serotonin transporter
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批准号:8477163
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项目类别:
-
资助金额:$26.82万
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财政年份:2009
-
负责人:JEFFRY D. MADURA
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依托单位:
CRCNS: Computational and experimental study of dopamine and serotonin transporter
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批准号:7771845
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项目类别:
-
资助金额:$30.91万
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财政年份:2009
-
负责人:JEFFRY D. MADURA
-
依托单位:
CRCNS: Computational and experimental study of dopamine and serotonin transporter
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批准号:8073152
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项目类别:
-
资助金额:$29.68万
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财政年份:2009
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负责人:JEFFRY D. MADURA
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依托单位:
CRCNS: Computational and experimental study of dopamine and serotonin transporter
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批准号:7869264
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项目类别:
-
资助金额:$30.6万
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财政年份:2009
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负责人:JEFFRY D. MADURA
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依托单位:
REU SUMMER PROGRAM AT DUQUESNE UNIVERSITY, SUMMER 2005
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批准号:7723199
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项目类别:
-
资助金额:$0.05万
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财政年份:2008
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负责人:JEFFRY D. MADURA
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依托单位:
REU SUMMER PROGRAM AT DUQUESNE UNIVERSITY, SUMMER 2005
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批准号:7601455
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项目类别:
-
资助金额:$0.03万
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财政年份:2007
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负责人:JEFFRY D. MADURA
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依托单位:
Force Field Effects on the Hydration of Type I Antifreeze Protein
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批准号:6980182
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项目类别:
-
资助金额:$0.11万
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财政年份:2004
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负责人:JEFFRY D. MADURA
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依托单位:
Marvel Friendly Grant
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批准号:6980233
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项目类别:
-
资助金额:$0.11万
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财政年份:2004
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负责人:JEFFRY D. MADURA
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依托单位:
QM and MM Simulations of Chemical and Biomolecular Systems
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批准号:6980171
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项目类别:
-
资助金额:$0.11万
-
财政年份:2004
-
负责人:JEFFRY D. MADURA
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依托单位:
QM AND MM SIMULATIONS OF CHEMICAL AND BIOMOLECULAR SYSTEMS
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批准号:7181696
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项目类别:
-
资助金额:$0.1万
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财政年份:2004
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负责人:JEFFRY D. MADURA
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依托单位:
MD Simulations of HIV-1 RT Complex and Binding Free Energy
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批准号:6980043
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项目类别:
-
资助金额:$0.55万
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财政年份:2004
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负责人:JEFFRY D. MADURA
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依托单位:
Computational Support for Critical Assesment of Structure Prediction (CASP) of
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批准号:6980070
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项目类别:
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资助金额:$0.11万
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财政年份:2004
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负责人:JEFFRY D. MADURA
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依托单位:
ENZYME & PROTEIN SIMULATIONS
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批准号:6319810
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项目类别:
-
资助金额:$0.13万
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财政年份:1999
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负责人:JEFFRY D. MADURA
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依托单位:--
STRUCTURE AND MECHANISMS OF FAMILY 18 CHITINASES
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批准号:6526012
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项目类别:
-
资助金额:$22.04万
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财政年份:1998
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负责人:JEFFRY D. MADURA
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依托单位:
STRUCTURE AND MECHANISMS OF FAMILY 18 CHITINASES
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批准号:6181484
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项目类别:
-
资助金额:$20.78万
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财政年份:1998
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负责人:JEFFRY D. MADURA
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依托单位:
STRUCTURE AND MECHANISMS OF FAMILY 18 CHITINASES
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批准号:2850574
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项目类别:
-
资助金额:$20.04万
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财政年份:1998
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负责人:JEFFRY D. MADURA
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依托单位:
STRUCTURE AND MECHANISMS OF FAMILY 18 CHITINASES
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批准号:6386501
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项目类别:
-
资助金额:$21.4万
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财政年份:1998
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负责人:JEFFRY D. MADURA
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依托单位:
STRUCTURE AND MECHANISMS OF FAMILY 18 CHITINASES
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批准号:6019527
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项目类别:
-
资助金额:$20.18万
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财政年份:1998
-
负责人:JEFFRY D. MADURA
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依托单位:
海外基金