MARKEY CENTER FOR STRUCTURAL BIOLOGY, PURDUE UNIVERSITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在普渡大学马基结构生物学中心，有九个小组使用X射线结晶学来了解各种生物过程的结构基础。作为一个集体，我们希望提交一份联合提案，以获得先进光子源光束线的访问权限。这项建议的目标是1)最大限度地利用APS资源，2)为研究生和博士后研究员提供培训。晶体数据的收集变得相当迅速，特别是在同步加速器源上。因此，许多实验室，特别是较小的实验室，发现很难完全填补光束线上的24小时轮班。为了节省这一宝贵的资源，我们提交了这项计划提案，允许普渡的团队在APS分班。这种操作模式还有一个额外的优势，即让来自不同实验室的学生和博士后研究员一起工作来收集他们的数据。这将促进信息和思想的交流，从而提高科学的质量，并为我们的学生和博士后实习生提供一个很好的学习机会。参与本意见书的私人投资者包括：陈觉、威廉·克雷默、芭芭拉·戈登、迈克尔·罗斯曼、辛西娅·施陶法赫、乔·卡波克和迪内什·耶尔努尔。 关于具有代表性的项目和以前GUP提案的进展情况，请参阅所附的pdf文件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Within the Markey Center for Structural Biology at Purdue University, there are nine groups using X-ray crystallography to understand the structural basis of a wide variety of biological processes. As a group, we would like to submit a joint proposal to gain access to the Advanced Photon Source beamlines. The goals of this proposal are to 1) make maximal use of the APS resource and 2) provide training to graduate students and postdoctoral fellows. Crystallographic data collection has become quite rapid, especially at synchrotron sources. As a result, many laboratories, especially the smaller laboratories, find it difficult to completely fill a 24 hour shift at a beamline. To conserve this valuable resource, we submit this program proposal that will allow the groups at Purdue to split shifts at APS. This mode of operation has the added advantage of having students and postdoctoral fellows from various laboratories working together to collect their data. This will facilitate an exchange of information and ideas, and thereby improve the quality of the science and provide an excellent learning opportunity for our student and postdoctoral trainees. Participating PIs in this submission are: Jue Chen, William Cramer, Barbara Golden, Michael Rossmann, Cynthia Stauffacher, Joe Kappock and Dinesh Yernool. Please see attached pdf for descriptions of representative projects and progress from the previous GUP proposal.