Quantitative Imaging for Biochemistry

描述（由申请人提供）：现代生物化学需要能够使用各种报告分子（包括荧光团和近红外范围染料）定量监测生物反应。此外，放射性标记提供了非常灵敏的检测手段，并且具有可以在不改变样品的化学组成的情况下引入它们的优点。在本提案中，我们要求提供资金，用于购买Typhoon FLA9000，这是一种多用途扫描仪，能够在各种情况下检测所有这些报告分子，包括聚丙烯酰胺凝胶，多孔板，薄层色谱板等。这是一款非常适合多用户设施的强大仪器。相关软件允许定量、背景校正和导出所采集的数据，以便可以在第三方电子表格或绘图程序中进行分析。该提案的长期目标是为NIH支持的研究人员提供关键基础设施。这台仪器将取代一台10年前的台风扫描仪，该扫描仪已经过时，很快就无法修复。新仪器将通过增加可用于监测生物反应的荧光团和染料的范围并提供更高的灵敏度来增强能力。

DESCRIPTION (provided by applicant): Modern biochemistry requires the ability to quantitatively monitor biological reactions using a wide variety of reporters, including fluorophores and near-IR range dyes. In addition, radiolabels provide a very sensitive means of detections and have the advantage that they can be introduced without varying the chemical makeup of the sample. In this proposal, we request funds for the purchase of a Typhoon FLA9000, a multipurpose scanner that is capable of detecting all of these reporters in a variety of contexts, including polyacrylamide gels, multiwell plates, thin layer chromatography plates and others. This is a robust instrument that is highly suited for a multiuser facility. The associated software allows quantitation, background correction and export of the acquired data such that it can be analyzed in third party spreadsheet or graphing programs. The long term objectives of this proposal are to provide critical infrastructure to NIH-supported researchers. This instrument will replace a 10 year old Typhoon scanner that is outmoded and will soon be unrepairable. The new instrument will enhance capability by increasing the range of fluorophores and dyes that can be used to monitor biological reactions and providing improved sensitivity.