STRUCTURAL ANALYSIS OF O-ANTIGEN FROM F TULARENSIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项合作的长期目标是确定F.可用作疫苗生产的保护性抗原的土拉热菌表面分子。 为了达到这些目的，我们对F.土拉菌脂多糖 这种O-抗原的结构是已知的核光谱证据的基础上的大部分性质的多糖。 此外，基于全甲基化分子的基质辅助激光解吸/电离，报告了与该结构一致的O-抗原组成。 在最初的研究中，我们正在对分离自强毒和无毒F.土拉菌菌株 我们将使用质谱资源开发的串联质谱方法详细描述这些结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long term goal of this collaboration is to determine the structures of F. tularensis surface molecules useful as protective antigens for vaccine production. Towards these ends we are characterizing the O-antigen portioni of the F. tularensis lipopolysaccharide. The structure of this O-antigen is known from nuclear spectroscopic evidence based on the bulk properties of the polysaccharide. In addition, the O-antigen composition consistent with this structure was reported based on matrix assisted laser desorption/ionization of the permethylated molecule. In initial studies, we are producing detailed mass profiles on O-antigen isolated from virulent and avirulent F. tularensis strains. We will characterize these structures in detail using tandem mass spectrometric methods developed at the Mass Spectrometry Resource.