LONGITUDINAL 1H NMR SPECTROSCOPY OF MOUSE HD

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是利用转基因小鼠模型研究亨廷顿病（HD）进展过程中神经化学的变化。亨廷顿氏病是一种遗传性神经退行性疾病，由某些脑区域的脑细胞的遗传程序性变性引起。基因操纵的老鼠，其DNA含有有缺陷的人类基因，被用来模拟这种人类大脑疾病。在高磁场下的体内1H NMR光谱用于非侵入性定量来自选定小鼠脑区域的代谢物。这种方法的非侵入性特征使得能够纵向监测神经变性背后的生化变化。本项目的目的是在分子水平上更好地了解HD神经退行性变的机制和过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to investigate the neurochemical changes occurring during the progression of the Huntington's disease (HD) using transgenic mouse models. Huntington's disease is an inherited neurodegenerative disease resulting from genetically programmed degeneration of brain cells in certain brain regions. Genetically manipulated mice, which DNA contain the defected human gene, are used to mimic this human brain disease. In vivo 1H NMR spectroscopy at high magnetic fields is used for a non-invasive quantification of metabolites from selected mouse brain regions. Non-invasive character of this method enables a longitudinal monitoring of biochemical changes underlying the neurodegeneration. The aim of this project is a better understanding of the mechanisms and processes of the neurodegeneration in HD on the molecular level.