BIODEGRADATION BY WHITE ROT PHANEROCHATE CHRYSOSPORIUM

白腐病金孢子菌的生物降解

• 批准号: 8168630
• 负责人: Paul A Langan
• 金额: $ 0.54万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168630
• 关键词: Biodegradation; Biological Process; Biomass; Cell Wall; Cellulose; Chemicals; Chrysosporium; Complex; Computer Retrieval of Information on Scientific Projects Database; Ecosystem; Enzymes; Event; Fiber; Funding; Grant; Institution; Lignin; Mass Spectrum Analysis; Nature; Phanerochaete chrysosporium; Plants; Research; Research Personnel; Resources; Sampling; Scanning; Source; Staging; Structure; Techniques; United States National Institutes of Health; Work; X ray diffraction analysis; X-Ray Diffraction; beamline; design; fungus; hemicellulose; insight; sugar; tool; white rot fungus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellulosic biomass, the inedible fibrous part of plant cell walls, has great potential as a source of sugars that could be used as platform chemicals to make fuels, materials and other chemicals. However, the complex structure and composition of biomass impedes its efficient degradation and conversion into sugars. Most sugars are locked in crystalline cellulose fibers which are sheathed in hemicellulose and encrusted in lignin. We are looking to nature to overcome this technical roadblock. Although many fungi degrade parts of biomass, white rot fungi are unique in their ability to degrade it completely. By using a combination of different techniques we hope to capture the degradation strategies of white rot fungus Phanerochaete chrysosporium on Poplar (a biomass source with commercial potential) and then to optimize for possible industrial application. Our major scientific objectives are to define the key metabolites and enzymes secreted by P.chrysosporium during degradation, using mass spectrometry, and then to relate these factors to specific degradation events in the Poplar samples. We hope to identify those specific degradation events by careful structural and compositional analysis of Poplar samples at different stages of fungal degradation. We are using scanning microprobe X-ray diffraction (SMX) using the BioCAT beamline 18ID as one of our tools to approach this problem, Our hope is that not only will this work advance our understanding of a biological process of fundamental importance to our ecosystem but that it will also provide insights that guide the design for industrial

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 纤维素生物质是植物细胞壁中不可食用的纤维部分，作为糖的来源具有巨大的潜力，可用作制造燃料，材料和其他化学品的平台化学品。然而，生物质的复杂结构和组成阻碍了其有效降解和转化为糖。大多数糖被锁定在结晶纤维素纤维中，结晶纤维素纤维被半纤维素包裹并被木质素包裹。我们正在寻求自然来克服这一技术障碍。尽管许多真菌降解部分生物质，但白色腐真菌在完全降解生物质的能力方面是独特的。通过不同技术的组合，我们希望捕捉白色腐真菌黄孢原毛革菌在白杨（一种具有商业潜力的生物质资源）上的降解策略，然后优化可能的工业应用。我们的主要科学目标是使用质谱法确定P.chrysosporium在降解过程中分泌的关键代谢物和酶，然后将这些因素与白杨样品中的特定降解事件联系起来。我们希望通过对真菌降解不同阶段的白杨样品进行仔细的结构和成分分析来确定这些特定的降解事件。我们正在使用扫描微探针X射线衍射（SMX），使用BioCAT光束线18 ID作为我们的工具之一来解决这个问题，我们希望这项工作不仅能促进我们对生态系统至关重要的生物过程的理解，而且还能提供指导工业设计的见解。