NANOSTRUCTURE OF PROTEIN/POLYSACCHARIDE COACERVATES DETERMINED BY SAXS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 近年来，蛋白质在生物电解质溶液中和生物电解质表面的纳米级自聚集现象受到了广泛的关注，这是由于其具有巨大的科学和技术意义，从药物/食品输送系统到纳米级生物传感器。然而，许多问题之间的关系，由蛋白质和生物聚电解质形成的复合物的结构，流变性能，化学组成，以及它们的分子间的相互作用是不清楚的，特别是在特定的结合离子，如K+，Cs+，和Ca 2+等的存在下，我们的总体研究目标是建立一个明确的结构，流变性能和化学组成之间的关系。作为我们总体研究目标的一部分，在这项具体的拟议研究中，我们希望研究理化参数的影响，如pH值，离子强度，蛋白质/淀粉比，蛋白质类型（β-乳球蛋白与牛肉明胶和鱼明胶）对蛋白质和生物聚电解质形成的复合物的结构的影响，例如具有不同官能团的藻酸盐，具有不同线性电荷密度的果胶，不同分子量的壳聚糖和羧甲基纤维素（CMC）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nanoscale self-aggregation of proteins in biopolyelectrolyte solutions and on biopolyelectrolyte surface has received considerable attention in recent years due to tremendous scientific and technological implications, ranging from drug/food delivery systems to nanoscale biosensors. However, many issues related to the relationship among structure, rheological properties, chemical composition of the complexes formed by proteins and biopolyelectrolytes, as well as their intermolecular interactions are not clear, especially in the presence of specific binding ions, such as K+, Cs+, and Ca2+ etc. Our overall research goal is to establish a clear relationship among structure, rheological properties, and chemical composition. As part of our overall research goal, in this specific proposed research, we expect to study effects of physicochemical parameters, such as pH, ionic strength, protein/polyelectrolyte ratio, type of proteins (beta-lactoglobulin vs. beef gelatin and fish gelatin) on the structure of complexes formed by proteins and biopolyelectrolytes, such as alginate with different functional groups, pectin with different linear charge densities, chitosan of different molecular weights, and carboxymethyl cellulose (CMC).