NANOSTRUCTURE OF PROTEIN/POLYSACCHARIDE COACERVATES DETERMINED BY SAXS

SAX 测定蛋白质/多糖凝聚物的纳米结构

• 批准号: 8168659
• 负责人: QIAN HUANG
• 金额: $ 0.54万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168659
• 关键词: Alginates; Attention; Binding; Biosensor; Carboxymethylcellulose; Charge; Chitosan; Complex; Computer Retrieval of Information on Scientific Projects Database; Fishes; Food; Funding; Gelatin; Goals; Grant; Institution; Ionic Strengths; Ions; Molecular Weight; Nanostructures; Pectins; Pharmaceutical Preparations; Polysaccharides; Proteins; Research; Research Personnel; Resources; Solutions; Source; Structure; Surface; System; United States National Institutes of Health; beef; beta Lactoglobulin; chemical property; density; functional group; intermolecular interaction; nanoscale; protein aggregation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nanoscale self-aggregation of proteins in biopolyelectrolyte solutions and on biopolyelectrolyte surface has received considerable attention in recent years due to tremendous scientific and technological implications, ranging from drug/food delivery systems to nanoscale biosensors. However, many issues related to the relationship among structure, rheological properties, chemical composition of the complexes formed by proteins and biopolyelectrolytes, as well as their intermolecular interactions are not clear, especially in the presence of specific binding ions, such as K+, Cs+, and Ca2+ etc. Our overall research goal is to establish a clear relationship among structure, rheological properties, and chemical composition. As part of our overall research goal, in this specific proposed research, we expect to study effects of physicochemical parameters, such as pH, ionic strength, protein/polyelectrolyte ratio, type of proteins (beta-lactoglobulin vs. beef gelatin and fish gelatin) on the structure of complexes formed by proteins and biopolyelectrolytes, such as alginate with different functional groups, pectin with different linear charge densities, chitosan of different molecular weights, and carboxymethyl cellulose (CMC).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 近年来，由于从药物/食品输送系统到纳米级生物传感器的巨大科学和技术影响，生物聚电解质溶液中和生物聚电解质表面上的蛋白质纳米级自聚集受到了相当大的关注。然而，蛋白质与生物聚电解质形成的复合物的结构、流变性质、化学成分之间的关​​系以及它们的分子间相互作用的许多问题尚不清楚，特别是在存在特定结合离子，如K+、Cs+和Ca2+等的情况下。我们的总体研究目标是建立结构、流变性质和化学成分之间的明确关系。作为我们总体研究目标的一部分，在这项具体的拟议研究中，我们希望研究物理化学参数的影响，例如pH、离子强度、蛋白质/聚电解质比率、蛋白质类型（β-乳球蛋白与牛肉明胶和鱼明胶）对蛋白质和生物聚电解质形成的复合物结构的影响，例如具有不同官能团的海藻酸盐、果胶 不同线电荷密度、不同分子量的壳聚糖、羧甲基纤维素(CMC)。