NANOSTRUCTURE OF PROTEIN/POLYSACCHARIDE COACERVATES DETERMINED BY SAXS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 近年来，从药物/食品递送系统到纳米生物传感器，蛋白质在生物聚电解质溶液和生物聚电解质表面的纳米尺度自聚集受到了极大的关注。然而，关于蛋白质与生物多电解质形成的络合物的结构、流变性、化学组成以及它们之间的相互作用等许多问题还不清楚，特别是在K+、Cs+、Ca~(2+)等特定结合离子存在的情况下，我们的总体研究目标是建立结构、流变性和化学组成之间的明确关系。作为我们总体研究目标的一部分，在这项拟议的具体研究中，我们希望研究物理化学参数，如pH、离子强度、蛋白质/聚电解质比、蛋白质类型(β-乳球蛋白与牛肉明胶和鱼胶)对蛋白质与生物多聚电解质形成的复合体结构的影响，如不同官能团的海藻酸盐、不同线电荷密度的果胶、不同相对分子质量的壳聚糖和羧甲基纤维素(CMC)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nanoscale self-aggregation of proteins in biopolyelectrolyte solutions and on biopolyelectrolyte surface has received considerable attention in recent years due to tremendous scientific and technological implications, ranging from drug/food delivery systems to nanoscale biosensors. However, many issues related to the relationship among structure, rheological properties, chemical composition of the complexes formed by proteins and biopolyelectrolytes, as well as their intermolecular interactions are not clear, especially in the presence of specific binding ions, such as K+, Cs+, and Ca2+ etc. Our overall research goal is to establish a clear relationship among structure, rheological properties, and chemical composition. As part of our overall research goal, in this specific proposed research, we expect to study effects of physicochemical parameters, such as pH, ionic strength, protein/polyelectrolyte ratio, type of proteins (beta-lactoglobulin vs. beef gelatin and fish gelatin) on the structure of complexes formed by proteins and biopolyelectrolytes, such as alginate with different functional groups, pectin with different linear charge densities, chitosan of different molecular weights, and carboxymethyl cellulose (CMC).