MAD USING MEDIUM ANGLE X-RAY SOLUTION SCATTERING (MADMAX)
使用中角 X 射线溶液散射 (MADMAX) 进行 MAD
基本信息
- 批准号:8168634
- 负责人:
- 金额:$ 2.15万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-01 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:AwardComputer Retrieval of Information on Scientific Projects DatabaseFundingGrantHemoglobinInstitutionIronLabelMeasurementMeasuresMethodsModelingPatternPotassium ChannelProteinsResearchResearch PersonnelResourcesRoentgen RaysSeleniumSelenomethionineSolutionsSourceStructureTestingUnited States National Institutes of HealthWaterWorkaqueousinnovationmacromoleculeprotein structureresearch studytechnique development
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
This project involves use of a new method, Multi-wavelength Anomalous Diffraction (MADMAX) using Medium Angle X-ray solution scattering (MADMAX), for the precise measurement of interatomic distances within proteins and other macromolecules in aqueous solution. Our preliminary experiments have shown that anomalous differences can be measured from proteins in solution. Differences from selenium scatter have been observed in solution scattering from the potassium channel, kcsa; differences from iron have been observed in scattering from hemoglobin. The work is supported by an NIH Eureka award (for highly innovative research) to support the development of this technique. Recently, we developed a method for computing wide-angle x-ray solution scattering (WAXS) from atomic coordinate sets using an explicit atom representation of water (Park et al.,2009). This, which requires no fitting of parameters) allows us to calculate WAXS patterns to within experimental error to test models for protein structure. We have now extended that capability to include calculation of the anomalous scattering components of proteins (Bardhan and Makowski, unpublished). This greatly increases the power of MADMAX, allowing us to predict the anomalous differences when there are multiple anomalous scattering atoms in the protein. Currently we are collecting anomalous scattering from selenomethionine-labelled proteins of known structure to determine the power of the method for proteins with 1 or 2 or 6-10 selenium labels.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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LEE MAKOWSKI其他文献
LEE MAKOWSKI的其他文献
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{{ truncateString('LEE MAKOWSKI', 18)}}的其他基金
Localization of fibrillar polymorphs in human brain tissue
人脑组织中纤维状多晶型物的定位
- 批准号:
10043200 - 财政年份:2020
- 资助金额:
$ 2.15万 - 项目类别:
WAXS AS A PROBE FOR THE STUDY OF PROTEIN STRUCTURE, DYNAMICS AND FUNCTION
蜡作为研究蛋白质结构、动力学和功能的探针
- 批准号:
8361272 - 财政年份:2011
- 资助金额:
$ 2.15万 - 项目类别:
WAXS AS A PROBE FOR THE STUDY OF PROTEIN STRUCTURE, DYNAMICS AND FUNCTION
蜡作为研究蛋白质结构、动力学和功能的探针
- 批准号:
8168619 - 财政年份:2010
- 资助金额:
$ 2.15万 - 项目类别:
MADMAX: PRECISE MEASUREMENT OF CONFORMATIONAL CHANGES IN PROTEINS
MADMAX:精确测量蛋白质构象变化
- 批准号:
7954934 - 财政年份:2009
- 资助金额:
$ 2.15万 - 项目类别:
Wide Angle X-ray Scattering for Commercial Applications in Drug Lead Discovery
广角 X 射线散射在先导药物发现中的商业应用
- 批准号:
7671745 - 财政年份:2009
- 资助金额:
$ 2.15万 - 项目类别:
X-RAY AND NEUTRON SCATTERING STUDIES OF COLLECTIVE MOTIONS IN PROTEINS
蛋白质集体运动的 X 射线和中子散射研究
- 批准号:
7954900 - 财政年份:2009
- 资助金额:
$ 2.15万 - 项目类别:
MADMAX: Precise Measurement of Conformational Changes in Proteins
MADMAX:精确测量蛋白质构象变化
- 批准号:
8230589 - 财政年份:2008
- 资助金额:
$ 2.15万 - 项目类别:
MADMAX: Precise Measurement of Conformational Changes in Proteins
MADMAX:精确测量蛋白质构象变化
- 批准号:
7515390 - 财政年份:2008
- 资助金额:
$ 2.15万 - 项目类别: