SIRNA AND DEVELOPMENT OF A MALE CONTRACEPTIVE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 这项试点提案的具体目的是确定狒狒睾丸中Ldhc的敲低是否会破坏精子功能，并作为男性避孕技术的基础。Goldberg和同事（Odet等人，Biol Reprod. 2008年7月; 79（1）：26-34。Epub 2008 Mar 26）成功地靶向破坏小鼠中的Ldhc基因。 这些雄性的精子不能使卵子受精。 这一观察结果证实LDHC是避孕发展的一个目标。 我们的合作者，耶鲁大学的Mark Saltzman博士已经能够证明siRNA可以用于生物可降解的递送系统中以敲低基因表达（个人交流），这表明以这种方式靶向Ldhc应该是一种有效的男性避孕药。 显然需要为男子提供创新的避孕技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this pilot proposal is to determine whether knockdown of Ldhc in the baboon testis can disrupt sperm function and serve as the basis for a male contraceptive technology. Goldberg and co-workers (Odet et al., Biol Reprod. 2008 Jul; 79(1):26-34. Epub 2008 Mar 26) were successful in targeting disruption of the Ldhc gene in mice. Sperm from these males were unable to fertilize eggs. This observation validates LDHC as a target for contraceptive development. Our collaborator, Dr. Mark Saltzman, Yale University has been able to demonstrate that siRNA can be utilized in a biodegradable delivery system to knock down gene expression (personal communication) suggesting that targeting Ldhc in this way should be an effective male contraceptive. There is an obvious need for innovative contraceptive technology for men.