SIRNA AND DEVELOPMENT OF A MALE CONTRACEPTIVE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该试点提案的具体目的是确定狒狒睾丸中 Ldhc 的敲低是否会破坏精子功能并作为男性避孕技术的基础。 Goldberg 及其同事（Odet 等人，Biol Reprod. 2008 Jul；79(1):26-34. Epub 2008 Mar 26）成功地靶向破坏小鼠的 Ldhc 基因。 这些雄性的精子无法使卵子受精。 这一观察结果证实了 LDHC 作为避孕药具开发的目标。 我们的合作者，耶鲁大学的 Mark Saltzman 博士已经能够证明 siRNA 可用于可生物降解的递送系统来抑制基因表达（个人交流），这表明以这种方式靶向 Ldhc 应该是一种有效的男性避孕药。 男性显然需要创新的避孕技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this pilot proposal is to determine whether knockdown of Ldhc in the baboon testis can disrupt sperm function and serve as the basis for a male contraceptive technology. Goldberg and co-workers (Odet et al., Biol Reprod. 2008 Jul; 79(1):26-34. Epub 2008 Mar 26) were successful in targeting disruption of the Ldhc gene in mice. Sperm from these males were unable to fertilize eggs. This observation validates LDHC as a target for contraceptive development. Our collaborator, Dr. Mark Saltzman, Yale University has been able to demonstrate that siRNA can be utilized in a biodegradable delivery system to knock down gene expression (personal communication) suggesting that targeting Ldhc in this way should be an effective male contraceptive. There is an obvious need for innovative contraceptive technology for men.