SIRNA AND DEVELOPMENT OF A MALE CONTRACEPTIVE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项试点计划的具体目的是确定敲除狒狒睾丸中的Ldhc基因是否会扰乱精子功能，并作为男性避孕技术的基础。Goldberg和同事(Odet等人，Biol Reprod.2008年7月；79(1)：26-34。EPub 2008-3月26)成功地在小鼠体内靶向破坏了Ldhc基因。这些雄性的精子不能使卵子受精。这一观察结果证实了LDHC是避孕开发的目标。我们的合作者，耶鲁大学的Mark Saltzman博士已经能够证明siRNA可以在可生物降解的递送系统中用于抑制基因表达(个人交流)，这表明以这种方式靶向Ldhc应该是一种有效的男性避孕药。男性显然需要创新的避孕技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific aim of this pilot proposal is to determine whether knockdown of Ldhc in the baboon testis can disrupt sperm function and serve as the basis for a male contraceptive technology. Goldberg and co-workers (Odet et al., Biol Reprod. 2008 Jul; 79(1):26-34. Epub 2008 Mar 26) were successful in targeting disruption of the Ldhc gene in mice. Sperm from these males were unable to fertilize eggs. This observation validates LDHC as a target for contraceptive development. Our collaborator, Dr. Mark Saltzman, Yale University has been able to demonstrate that siRNA can be utilized in a biodegradable delivery system to knock down gene expression (personal communication) suggesting that targeting Ldhc in this way should be an effective male contraceptive. There is an obvious need for innovative contraceptive technology for men.