MHC TYPING OF MACAQUES USED IN AIDS RESEARCH

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 目的：为猕猴（印度恒河猴、中国恒河猴和食蟹猴）的MHC Ⅰ类和Ⅱ类基因座分型提供依据。使HLA分型技术适用于猕猴（印度恒河猴、中国恒河猴和食蟹猴）MHC I类和II类分子分型。 在HIV疫苗开发和发病机制研究中，各种猕猴作为动物模型的实用性越来越高，需要继续参考这些物种的MHC分型实验室。我们计划继续为北美和欧洲科学界提供猕猴MHC分型服务。最初，这将包括对编码MHC I类和II类分子的等位基因进行PCR-SSP测试，这些分子结合来自SIV和SHIV的肽。我们正在开发用于分析印度恒河猴、中国恒河猴和食蟹猴MHC I类和II类等位基因的其他分子技术。此外，我们为希望建立MHC分型的各个实验室提供培训和材料。最后，我们正在开发一组特征良好的细胞系，这将是非常宝贵的猕猴MHC的分析。自2002年至今，我们已为50个机构的130多名研究人员进行了超过126，000次打字。2009年，我们应来自19个不同机构的23名主要研究人员的要求进行了21，800次打字。我们正准备在2010年提供扩大的II类打字服务。 这项研究使用WNPRC免疫遗传学和病毒学服务。 出版物： Doxiadis GG，Heijmans CM，Bonhomme M，Otting N，Crouau-Roy B，Bontrop RE.通过微卫星分析和逆转录病毒序列定位揭示恒河猴MHC I类B区的复合进化历史。PLoS One. 2009; 4（1）：e4287. PMID：19172173 Wilson NA，Keele BF，Reed JS，Piaskowski SM，MacNair CE，Bett AJ，Liang X，Wang F，Thoryk E，Heidecker GJ，Citron MP，Huang L，Lin J，Vitelli S，Ahn CD，Kaizu M，Maness NJ，Reynolds MR，Friedrich TC，Loffredo JT，Rakasz EG，Erickson S，Allison DB，Piatak M Jr，Lifson JD，Shiver JW，Casimiro DR，Shaw GM，Hahn BH，Watkins DI.疫苗诱导的细胞反应控制异源攻击后猴免疫缺陷病毒的复制。J Virol。2009年7月; 83（13）：6508 - 21. PMID：19403685 Loffredo JT，Sidney J，Bean AT，比尔DR，Bardet W，Wahl A，Hawkins OE，Piaskowski S，Wilson NA，Hildebrand WH，Watkins DI，Sette A.与免疫缺陷病毒复制的精英控制相关的两种MHC I类分子Mamu-B * 08和HLA-B * 2705结合具有序列相似性的肽。 免疫学杂志2009年6月15日; 182（12）：7763 - 75。PMID：19494300 Valentine LE，Loffredo JT，Bean AT，Le ên EJ，MacNair CE，Beal DR，Piaskowski SM，Klimentidis YC，Lank SM，Wiseman RW，Weinfurter JT，May GE，Rakasz EG，Wilson NA，Friedrich TC，O'Connor DH，Allison DB，Watkins DI. "逃逸"病毒变异体感染削弱了对猴免疫缺陷病毒SIVmac239在Mamu-B * 08阳性猕猴中复制的控制。J Virol。2009年11月; 83（22）：11514 - 27。9月2.PMID：19726517 Vojnov L，Reed JS，Weisgrau KL，Rakasz EG，Loffredo JT，Piaskowski SM，Sacha JB，Kolar HL，Wilson NA，约翰逊RP，Watkins DI.有效的猴免疫缺陷病毒特异性CD8 + T细胞缺乏一个容易检测的，共享的特征。J Virol。2010年1月; 84（2）：753 - 64.PMID：19889785 杨伟，王伟，王伟. 纯种食蟹猴（Macaca fascicularis）Mafa-A和-B单倍型的定义免疫遗传学。2009年11月24日。PMID：19937015 de Groot NG，Heijmans CM，de Groot N，Doxiadis GG，Otting N，Bontrop RE. 黑猩猩Mhc-DRB区域再访：基因内容，多态性，假基因和成绩单。分子免疫学。2009年12月; 47（2 - 3）：381 - 9。PMID：19800692 Hessell AJ，Rakasz EG，Tehrani DM，Huber M，Weisgrau KL，Landucci G，Forthal DN，Koff WC，Poignard P，Watkins DI，Burton DR.针对人类免疫缺陷病毒1型gp41膜近端外部区域的广泛中和单克隆抗体2F5和4E10保护免受猴-人类免疫缺陷病毒SHIVBa-L的粘膜攻击。J Virol。2010年2月; 84（3）：1302 - 13。PMID：19906907

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To offer MHC typing of the MHC class I and II loci for investigators working with macaques (Indian rhesus, Chinese rhesus, and Cynomolgus). To adapt technologies for HLA typing to molecular typing of macaque (Indian rhesus, Chinese rhesus, and Cynomolgus) MHC class I and II. The increased utility of various species of macaques as animal models in both HIV vaccine development and pathogenesis studies necessitates the continuation of reference MHC typing laboratories for these species. We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. Initially, this will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV and SHIV. We are developing additional molecular techniques for analysis of the Indian rhesus and Chinese rhesus and Cynomolgus macaque MHC class I and class II alleles. Additionally, we offer training and materials to individual laboratories that wish to set up MHC typing. Finally, we are developing a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. Since 2002 to the present, we have performed over 126,000 typings for over 130 investigators at 50 institutions. In 2009, we have conducted 21,800 typings at the request of 23 principal investigators from 19 different institutions. We are preparing to offer expanded Class II typing services during 2010. This research used WNPRC Immunogenetics & Virology Services. PUBLICATIONS: Doxiadis GG, Heijmans CM, Bonhomme M, Otting N, Crouau-Roy B, Bontrop RE. Compound evolutionary history of the rhesus macaque MHC class I B region revealed by microsatellite analysis and localization of retroviral sequences. PLoS One. 2009;4(1):e4287. PMID: 19172173 Wilson NA, Keele BF, Reed JS, Piaskowski SM, MacNair CE, Bett AJ, Liang X, Wang F, Thoryk E, Heidecker GJ, Citron MP, Huang L, Lin J, Vitelli S, Ahn CD, Kaizu M, Maness NJ, Reynolds MR, Friedrich TC, Loffredo JT, Rakasz EG, Erickson S, Allison DB, Piatak M Jr, Lifson JD, Shiver JW, Casimiro DR, Shaw GM, Hahn BH, Watkins DI. Vaccine-induced cellular responses control simian immunodeficiency virus replication after heterologous challenge. J Virol. 2009 Jul;83(13):6508-21. PMID: 19403685 Loffredo JT, Sidney J, Bean AT, Beal DR, Bardet W, Wahl A, Hawkins OE, Piaskowski S, Wilson NA, Hildebrand WH, Watkins DI, Sette A. Two MHC class I molecules associated with elite control of immunodeficiency virus replication, Mamu-B*08 and HLA-B*2705, bind peptides with sequence similarity. J Immunol. 2009 Jun 15;182(12):7763-75. PMID: 19494300 Valentine LE, Loffredo JT, Bean AT, Le¿n EJ, MacNair CE, Beal DR, Piaskowski SM, Klimentidis YC, Lank SM, Wiseman RW, Weinfurter JT, May GE, Rakasz EG, Wilson NA, Friedrich TC, O'Connor DH, Allison DB, Watkins DI. Infection with "escaped" virus variants impairs control of simian immunodeficiency virus SIVmac239 replication in Mamu-B*08-positive macaques. J Virol. 2009 Nov;83(22):11514-27. Sep 2.PMID: 19726517 Vojnov L, Reed JS, Weisgrau KL, Rakasz EG, Loffredo JT, Piaskowski SM, Sacha JB, Kolar HL, Wilson NA, Johnson RP, Watkins DI. Effective simian immunodeficiency virus-specific CD8+ T cells lack an easily detectable, shared characteristic. J Virol. 2010 Jan;84(2):753-64.PMID: 19889785 Otting N, Doxiadis GG, Bontrop RE. Definition of Mafa-A and -B haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis). Immunogenetics. 2009 Nov 24. PMID: 19937015 de Groot NG, Heijmans CM, de Groot N, Doxiadis GG, Otting N, Bontrop RE. The chimpanzee Mhc-DRB region revisited: gene content, polymorphism, pseudogenes, and transcripts. Mol Immunol. 2009 Dec;47(2-3):381-9. PMID: 19800692 Hessell AJ, Rakasz EG, Tehrani DM, Huber M, Weisgrau KL, Landucci G, Forthal DN, Koff WC, Poignard P, Watkins DI, Burton DR. Broadly neutralizing monoclonal antibodies 2F5 and 4E10 directed against the human immunodeficiency virus type 1 gp41 membrane-proximal external region protect against mucosal challenge by simian-human immunodeficiency virus SHIVBa-L. J Virol. 2010 Feb;84(3):1302-13. PMID: 19906907