DEVELOPMENT OF IMMUNE MONITORING REAGENTS AND MHC TYPING TECHNOLOGIES

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目的：建立食蟹猴主要组织相容性复合体（MHC）分型技术 猕猴（Macaca fascicularis）和印度恒河猴（Macaca mulatta）。我们 将人类HLA分型技术应用于MHC I类和II类分型 在猕猴体内 进展： 从7只印度恒河猴中构建cDNA文库。这些动物 的MHC I类和II类基因的特征。十一小说 从这些动物中鉴定了MHC等位基因。每本小说至少两本 在两个方向上对等位基因进行全长测序，适当命名并提交给 Genbank和免疫多态性数据库。此外，11个新的稳定 从克隆的MHC等位基因产生表达转染子细胞系。 出版物： 所罗门C，Southwood S，Hoof I，Rudersdorf R，Peters B，Sidney J，Pinilla C， Marcondes MC，Ling B，马克思P，Sete A，Mothe BR.最常见的中国恒河猴 猕猴MHC I类分子与HLA-B7共享肽结合库 超类型免疫遗传学。2010年7月;62（7）：451-64。 PMID：20480161，PMCID： PMC2890073。 Southwood S，所罗门C，Hoof I，Rudersdorf R，Sidney J，Peters B，Wahl A，Hawkins 放大图片作者：A.频繁表达的功能分析 中国猕猴MHC I类。免疫遗传学。2011年1月28日[Epub领先于 打印]。PMID：21274527。 注：与艾滋病有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To develop and implement MHC typing technologies for Cynomolgus macaques (Macaca fascicularis) and Indian Rhesus macaques (Macaca mulatta). We will adapt technologies from HLA typing in humans for MHC class I and class II typing in the macaque. PROGRESS: cDNA libraries were constructed from seven Indian Rhesus macaques. These animals were completely characterized for their MHC class I and class II genes. Eleven novel MHC alleles were identified from these animals. At least two copies of each novel allele were full length sequenced in both directions, properly named and submitted to Genbank and the Immuno Polymorphism Database. Additionally, 11 new stably expressing transfectant cell lines were produced from cloned MHC alleles. PUBLICATIONS: Solomon C, Southwood S, Hoof I, Rudersdorf R, Peters B, Sidney J, Pinilla C, Marcondes MC, Ling B, Marx P, Sette A, Mothe BR. The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertype. Immunogenetics. 2010 Jul;62(7):451-64. PMID: 20480161, PMCID: PMC2890073. Southwood S, Solomon C, Hoof I, Rudersdorf R, Sidney J, Peters B, Wahl A, Hawkins O, Hildebrand W, Moth¿ BR, Sette A. Functional analysis of frequently expressed Chinese rhesus macaque MHC class I. Immunogenetics. 2011 Jan 28. [Epub ahead of print]. PMID: 21274527. Note: AIDS related.