DEVELOPMENT OF IMMUNE MONITORING REAGENTS AND MHC TYPING TECHNOLOGIES
DEVELOPMENT OF IMMUNE MONITORING REAGENTS AND MHC TYPING TECHNOLOGIES
批准号:
8358206
负责人:
David I Watkins
金额:
$63.15万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-05-01 至 2012-04-30
关键词:
Acquired Immunodeficiency SyndromeAllelesAnimalsBindingCell LineChinese PeopleDatabasesDevelopmentFundingGenbankGenetic PolymorphismGrantHLA-B7 AntigenHistocompatibility Antigens Class IHoofHumanImmunogeneticsImmunologic MonitoringLengthMHC Class I GenesMHC Class II GenesMacacaMacaca fascicularisMacaca mulattaNamesNational Center for Research ResourcesPeptidesPrimatesPrincipal InvestigatorPrintingReagentResearchResearch InfrastructureResourcesSourceTechnologyUnited States National Institutes of HealthWisconsincDNA Librarycostnovel
中文摘要
这个子项目是利用这些资源的众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Objective: To develop and implement MHC typing technologies for Cynomolgus
macaques (Macaca fascicularis) and Indian Rhesus macaques (Macaca mulatta). We
will adapt technologies from HLA typing in humans for MHC class I and class II typing
in the macaque.
PROGRESS:
cDNA libraries were constructed from seven Indian Rhesus macaques. These animals
were completely characterized for their MHC class I and class II genes. Eleven novel
MHC alleles were identified from these animals. At least two copies of each novel
allele were full length sequenced in both directions, properly named and submitted to
Genbank and the Immuno Polymorphism Database. Additionally, 11 new stably
expressing transfectant cell lines were produced from cloned MHC alleles.
PUBLICATIONS:
Solomon C, Southwood S, Hoof I, Rudersdorf R, Peters B, Sidney J, Pinilla C,
Marcondes MC, Ling B, Marx P, Sette A, Mothe BR. The most common Chinese rhesus
macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7
supertype. Immunogenetics. 2010 Jul;62(7):451-64. PMID: 20480161, PMCID:
PMC2890073.
Southwood S, Solomon C, Hoof I, Rudersdorf R, Sidney J, Peters B, Wahl A, Hawkins
O, Hildebrand W, Moth¿ BR, Sette A. Functional analysis of frequently expressed
Chinese rhesus macaque MHC class I. Immunogenetics. 2011 Jan 28. [Epub ahead of
print]. PMID: 21274527.
Note: AIDS related.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
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批准号:10669613
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项目类别:
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资助金额:$97.87万
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财政年份:2021
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负责人:David I Watkins
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依托单位:
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依托单位:
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批准号:10463875
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资助金额:$98.85万
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财政年份:2021
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依托单位:
Can vaccine-induced CD8 T cells prevent chronic phase AIDS virus replication?
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批准号:8787712
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项目类别:
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资助金额:$61.94万
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财政年份:2014
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负责人:David I Watkins
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依托单位:
Can vaccine-induced CD8 T cells prevent chronic phase AIDS virus replication?
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批准号:8976140
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项目类别:
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资助金额:$53.0万
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财政年份:2014
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负责人:David I Watkins
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依托单位:
Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development
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批准号:8497605
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项目类别:
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资助金额:$214.55万
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财政年份:2012
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负责人:David I Watkins
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依托单位:
Protective Immunity
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批准号:8307106
-
项目类别:
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资助金额:$51.45万
-
财政年份:2012
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负责人:David I Watkins
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依托单位:
Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development
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批准号:8688135
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项目类别:
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资助金额:$194.7万
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财政年份:2012
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负责人:David I Watkins
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依托单位:
Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development
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批准号:8301117
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项目类别:
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资助金额:$215.84万
-
财政年份:2012
-
负责人:David I Watkins
-
依托单位:
Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development
-
批准号:8874851
-
项目类别:
-
资助金额:$195.69万
-
财政年份:2012
-
负责人:David I Watkins
-
依托单位:
A NOVEL, LOGICAL APPROACH TO HIV VACCINE DEVELOPMENT
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批准号:8358204
-
项目类别:
-
资助金额:$23.83万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
IMMUNOGENICITY AND PROTECTION OF LIVE ATTENUATED SIV239 DELTA NEF IN RHESUS
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批准号:8358203
-
项目类别:
-
资助金额:$11.91万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
MINIGENE VACCINATION WITH EARLY PRESENTED VIRAL PROTEINSAIDS RELATED RESEARCH
-
批准号:8358214
-
项目类别:
-
资助金额:$38.13万
-
财政年份:2011
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负责人:David I Watkins
-
依托单位:
SIV-specific Mamu-E-restricted CD8+ T cells
-
批准号:8071437
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项目类别:
-
资助金额:$20.43万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
CRYPTIC ORFS AS A VACCINE FOR HIV
-
批准号:8358237
-
项目类别:
-
资助金额:$5.96万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
PROTECTIVE EFFICACY OF MERCK AD5 PRIME/BOOST AGAINST MUCOSAL CHALLENGE
-
批准号:8358247
-
项目类别:
-
资助金额:$17.87万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
VACCINE REGIMENS TO INDUCE CD4+ AND CD8+ T CELLS AGAINST SIV EPITOPES
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批准号:8358223
-
项目类别:
-
资助金额:$9.53万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
SIV-specific Mamu-E-restricted CD8+ T cells
-
批准号:8212162
-
项目类别:
-
资助金额:$21.64万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
MHC TYPING OF MACAQUES USED IN AIDS RESEARCH
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批准号:8358202
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项目类别:
-
资助金额:$28.59万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
MHC-BOUND, SIV-DERIVED, CTL AND HTL EPITOPES
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批准号:8358192
-
项目类别:
-
资助金额:$47.66万
-
财政年份:2011
-
负责人:David I Watkins
-
依托单位:
海外基金