DEVELOPMENT OF IMMUNE MONITORING REAGENTS AND MHC TYPING TECHNOLOGIES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目的：开发和实施食蟹猴的MHC分型技术 短尾猴(Macaca Fascularis)和印度恒河猴(Macaca Mulatta)。我们 将采用人类人类白细胞抗原分型技术进行MHC I类和II类分型 在猕猴身上。 进度： 以7只印度恒河猴为材料，构建了它们的基因文库。这些动物 完全具有MHC I类和II类基因的特征。十一部长篇小说 从这些动物中鉴定出MHC等位基因。每本小说至少两本 对等位基因进行了两个方向的全长测序，正确命名并提交给 GenBank和免疫多态数据库。此外，11个新的稳定 从克隆的MHC等位基因中获得了表达MHC的转基因细胞系。 出版物： 所罗门·C，索斯伍德·S，胡夫·I，鲁德斯多夫·R，彼得斯·B，西德尼·J，皮尼拉·C， Marcondes MC，Ling B，Max P，Sette A，Mothe BR。最常见的中国恒河猴 猕猴MHC-I类分子与人类白细胞抗原B7具有相同的多肽结合谱 超型。免疫遗传学。2010年7月；62(7)：451-。PMID：20480161，PMCID： PMC2890073。 索斯伍德·S、所罗门·C、胡夫一世、鲁德斯多夫·R、西德尼·J、彼得斯·B、瓦尔·A、霍金斯 O，Hildebrand W，Moth？Br，Sette A.频繁表达的功能分析 中国恒河猴MHC-I类免疫遗传学。2011年1月28日[ePub领先于 打印]。21274527。 注：与艾滋病有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To develop and implement MHC typing technologies for Cynomolgus macaques (Macaca fascicularis) and Indian Rhesus macaques (Macaca mulatta). We will adapt technologies from HLA typing in humans for MHC class I and class II typing in the macaque. PROGRESS: cDNA libraries were constructed from seven Indian Rhesus macaques. These animals were completely characterized for their MHC class I and class II genes. Eleven novel MHC alleles were identified from these animals. At least two copies of each novel allele were full length sequenced in both directions, properly named and submitted to Genbank and the Immuno Polymorphism Database. Additionally, 11 new stably expressing transfectant cell lines were produced from cloned MHC alleles. PUBLICATIONS: Solomon C, Southwood S, Hoof I, Rudersdorf R, Peters B, Sidney J, Pinilla C, Marcondes MC, Ling B, Marx P, Sette A, Mothe BR. The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertype. Immunogenetics. 2010 Jul;62(7):451-64. PMID: 20480161, PMCID: PMC2890073. Southwood S, Solomon C, Hoof I, Rudersdorf R, Sidney J, Peters B, Wahl A, Hawkins O, Hildebrand W, Moth¿ BR, Sette A. Functional analysis of frequently expressed Chinese rhesus macaque MHC class I. Immunogenetics. 2011 Jan 28. [Epub ahead of print]. PMID: 21274527. Note: AIDS related.