SPF INDIAN RHESUS MONKEY COLONY FOR AIDS RESEARCH: HIV ANIMAL MODELS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本申请的具体目的是建立无猿猴免疫缺陷病毒（SIV）、猿猴逆转录病毒（SRV）、疱疹B病毒和猿猴嗜t淋巴细胞病毒（STLV 1）的印度恒河猴群体。这将通过在第一年招募100只无特定病原体（SPF）雌性恒河猴和15只SPF雄性恒河猴来完成。在随后的4年中，每年将另外50只SPF雌性和10只雄性添加到建立群体中。我们预计，到赠款期结束时，殖民地将有625只动物。在前两年对猴进行4次特定病原体筛查，之后每年筛查2次。社会群体将保持较小，5至7只雌性，猴子将被安置在户外，在自然表面上的20英尺x20英尺的围栏中。将在该中心进行特定病原体的筛查，并利用商业实验室和NIH资助的检测中心对检测进行验证。我们将采用人工授精等辅助生殖技术，以最大限度地提高殖民地的生产力。此外，我们还与已建立的实验室签订合同，对殖民地的遗传多样性进行遗传监测，并确定目前在艾滋病研究中有价值的具体特征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this application is to establish a colony of Indian rhesus monkeys, free of Simian Immunodeficiency Virus (SIV), simian retrovirus (SRV), herpes b virus, and simian t-lymphotrophic virus (STLV1). This will be accomplished by recruitment of 100 specific pathogen free (SPF) female rhesus monkeys and 15 SPF males in the first year. An additional 50 SPF females and 10 males will be added to the founding colony in each of the subsequent 4 years. We project that by the end of the grant period the colony will have 625 animals. Monkeys will be screened for the presence of the specific pathogens 4 times in the first two years and twice a year after. Social groups will be kept small, 5 to 7 females, and the monkeys will be housed outdoors, in 20ft x 20ft enclosures on natural surfaces. Screening for the presence of the specific pathogens will be done at the Center with validation of assays utilizing commercial labs and NIH funded testing centers. We will incorporate assisted reproductive techniques such as artificial insemination to maximize the productivity of the colony. In addition, we have contracted with established laboratories to genetically monitor the colony for genetic diversity and to identify specific traits that are currently valuable in AIDS research.