SPF INDIAN RHESUS MONKEY COLONY FOR AIDS RESEARCH: HIV ANIMAL MODELS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项应用的具体目的是建立一个印度恒河猴群体，没有猿猴免疫缺陷病毒(SIV)、猿猴逆转录病毒(SRV)、疱疹B病毒和猿猴T淋巴营养病毒(STLV1)。这将通过在第一年招募100只无特定病原体(SPF)的雌性恒河猴和15只SPF雄性恒河猴来实现。在接下来的4年里，每年都会有另外50只SPF雌性和10只雄性被添加到创始殖民地。我们预计，到授权期结束时，该群体将拥有625只动物。猴子将在头两年接受4次特定病原体的筛查，并在之后的一年中进行两次筛查。社会团体将保持较小的规模，5至7只雌性，猴子将被安置在户外，在自然表面上20英尺x 20英尺的围栏里。该中心将利用商业实验室和NIH资助的检测中心对检测进行验证，以筛选特定病原体的存在。我们将采用人工授精等辅助生殖技术，以最大限度地提高种群的生产力。此外，我们还与现有的实验室签订了合同，对该群体的遗传多样性进行遗传监测，并确定目前在艾滋病研究中有价值的特定特征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this application is to establish a colony of Indian rhesus monkeys, free of Simian Immunodeficiency Virus (SIV), simian retrovirus (SRV), herpes b virus, and simian t-lymphotrophic virus (STLV1). This will be accomplished by recruitment of 100 specific pathogen free (SPF) female rhesus monkeys and 15 SPF males in the first year. An additional 50 SPF females and 10 males will be added to the founding colony in each of the subsequent 4 years. We project that by the end of the grant period the colony will have 625 animals. Monkeys will be screened for the presence of the specific pathogens 4 times in the first two years and twice a year after. Social groups will be kept small, 5 to 7 females, and the monkeys will be housed outdoors, in 20ft x 20ft enclosures on natural surfaces. Screening for the presence of the specific pathogens will be done at the Center with validation of assays utilizing commercial labs and NIH funded testing centers. We will incorporate assisted reproductive techniques such as artificial insemination to maximize the productivity of the colony. In addition, we have contracted with established laboratories to genetically monitor the colony for genetic diversity and to identify specific traits that are currently valuable in AIDS research.