Electron Microscopy Core

电子显微镜核心

• 批准号: 8179630
• 负责人: NIKOLAUS GRIGORIEFF
• 金额: $ 24.35万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8179630
• 关键词: Antibodies; Antigen-Antibody Complex; Automation; B cell repertoire; Binding; Collaborations; Complement; Complex; Data; Electron Microscope; Electron Microscopy; Epitope Mapping; Epitopes; Glycoproteins; Goals; Grant; Guns; Housing; Image; Immune response; Infection; Influenza; Instruction; Laboratories; Methods; Procedures; Process; Publications; Research; Resolution; Resources; Sampling; Screening procedure; Services; Structure; Three-Dimensional Image; Vaccines; Viral; Viral Proteins; Virion; Work; design; image processing; influenzavirus; instrument; instrumentation; neutralizing antibody; reconstruction; scaffold

PROJECT SUMMARY (See instructions): The electron microscopy core (EM Core) houses and maintains all the instrumentation, in particular an FEI Tecnai F30 electron microscope with a field emission gun operating at 300 kV, needed for the recording of high-resolution images of complexes between influenza virus hemagglutin (HA) and the Fabs of neutralizing antibodies. These complexes will be analyzed either as symmetrical displays on an icosahedral scaffold or as HA rosettes.. It also contains another high-resolution instrument (an FEI Tecnai F20 that will be installed at Brandeis during the next 12 months) and two smaller instruments for sample screening. The electron microscopes will be maintained by Dr. Chen Xu (Brandeis). Furthermore, the EM Core provides computing facilities to process the image data and produce high-quality 3D reconstructions. An image processing pipeline for medium throughput processing of these structures will be set up by Dr. James Chen (Brandeis).

项目总结（见说明）： 电子显微镜核心（EM Core）容纳并维护所有仪器，特别是具有在300 kV下操作的场发射枪的FEI Tecnai F30电子显微镜，其需要记录流感病毒血凝素（HA）和中和抗体Fab之间的复合物的高分辨率图像。这些复合物将作为二十面体支架上的对称展示物或作为HA载体进行分析。它还包括另一台高分辨率仪器（FEI Tecnai F20，将在未来12个月内安装在布兰代斯）和两台较小的仪器，用于样品筛选。电子显微镜将由Dr. Chen Xu（Brandeis）维护。此外，EM Core还提供计算设施来处理图像数据并生成高质量的3D重建。James Chen博士（Brandeis）将建立用于这些结构的中等吞吐量处理的图像处理管道。