A Tissue Implantable Microbiosensor

组织植入式微生物传感器

• 批准号: 8393149
• 负责人: Peter A Petillo
• 金额: $ 15万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8393149
• 关键词: American; Animals; Biosensor; Blood capillaries; Brain; Brain region; Choline; Communities; Computer software; Deposition; Detection; Development; Diagnosis; Disease; Electronics; Elements; Environment; Enzymes; Equipment; Ethanol; Foundations; Glucose; Glutamates; Goals; Heart Diseases; Histamine; Hospitalization; Human; Hydrogen Peroxide; Kansas; Laboratories; Lead; Letters; Literature; Malignant Neoplasms; Manufactured Materials; Manufacturer Name; Marketing; Measurement; Methods; Microdialysis; Monitor; Oxygen; Peroxides; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Platinum; Positioning Attribute; Prevention; Procedures; Protein Engineering; Proteins; Quality of life; Reporting; Reproducibility; Research; Research Personnel; Resolution; Resources; Sales; Science; Scientist; Serine; Specificity; Surface; System; Techniques; Technology; Testing; Tissues; Translating; United States; Universities; Urate; Work; ascorbate; base; biomaterial compatibility; capillary; cost; design; economic cost; experience; glucose sensor; in vivo; innovation; interest; meetings; nanofabrication; nanoparticle; nervous system disorder; novel; novel strategies; pre-clinical; professor; prototype; research study; response; success; tool

DESCRIPTION (provided by applicant): The aim of this application is to develop, prototype, validate, manufacture and commercialize a new, generalized microbiosensor designed for non-human use and based on functionalized nanoparticles. The microbiosensors produced will enable in vivo monitoring of a wide range of non-electroactive, neurologically relevant analytes, including, but not limited to, glucose, glutamate, lactate, choline, histamine, D-serine, and ethanol. The design can be extended to multiple conjoined capillaries to provide an array of biosensors for the simultaneous monitoring of multiple analytes, with high temporal and spatial resolution, which causes minimal damage to the surrounding tissue. The specific aim for Phase I is to demonstrate feasibility by prototyping and characterizing a tissue implantable enzyme functionalized nanoparticle microbiosensor that is suitable for in vivo brain measurements and extensible to multianalyte microbiosensor arrays. The proposed microbiosensor will allow researchers to target smaller subregions of the brain that would be damaged by currently available probes. This, in turn, will lead to a better understanding of function and assist in quantifying drug response. The proposed microbiosensor design can also be adapted to the simultaneous monitoring of multiple analytes within a single region of space. Turn-key tools that allow an enhanced level of specificity and granularity will be widely useful in research on the causes, prevention, diagnosis, and treatment of neurological disorders, and this will lead to new discoveries. Pinnacle will lead this application and work in conjunction with an interdisciplinary consortium of three leading scientists at the University of Kansas. Professor Judy Wu possesses extensive experience in materials science and nanofabrication techniques, Professor Mark Richter is skilled in protein engineering, and Professor George Wilson brings over 30 years of experience in biosensor development to the project. The facilities and equipment available at Pinnacle, and the various University of Kansas laboratories, will provide the resources required to successfully complete this project. Innovative aspects of this application include the use of active enzyme functionalized nanoparticles as the sensing element, and the controlled electrophoretic deposition of these nanoparticles. Also novel is a design that maximizes oxygen reuse and hydrogen peroxide detection efficiency. This will lead to linear microbiosensors with high sensitivity for the analyte being monitored. Extensive preliminary results are presented in the application. These results are the foundation for this Phase I effort. Overall, worldwide biosensor sales in FY2009 were $6.9 billion with 31 percent of the sales due to human glucose sensors. Pinnacle is an established manufacturer of biosensors and is well positioned to introduce this new class of biosensors to a broad pre-clinical market. PUBLIC HEALTH RELEVANCE: In the United States, over 1000 disorders of the brain and nervous system result in more hospitalizations than for any other disease group including heart disease and cancer. The quality of life, and economic, cost of brain and nervous system related disorders, is staggering. These disorders disrupt the lives of more than 50 million Americans each year and costs exceed $400 billion.

描述(由申请人提供)：本申请的目的是开发、原型、验证、制造和商业化一种非人类使用的、基于功能化纳米颗粒的新型通用微生物传感器。生产的微生物传感器将能够在体内监测一系列非电活性的神经学相关分析物，包括但不限于葡萄糖、谷氨酸、乳酸、胆碱、组胺、D-丝氨酸和乙醇。该设计可以扩展到多个连接的毛细管，以提供一系列生物传感器，用于同时监测多个分析物，具有高时间和空间分辨率，对周围组织造成的损害最小。第一阶段的具体目标是通过原型和表征一种组织可植入的酶功能化纳米颗粒微生物传感器来证明其可行性，该传感器适用于在体脑测量，并可扩展到多分析微生物传感器阵列。拟议的微生物传感器将使研究人员能够瞄准大脑中可能被目前可用的探针破坏的较小的亚区。反过来，这将导致对功能的更好理解，并有助于量化药物反应。所提出的微生物传感器设计也可用于在单个空间区域内同时监测多个分析物。允许提高特异性和粒度的交钥匙工具将在神经疾病的原因、预防、诊断和治疗的研究中广泛使用，这将导致新的发现。顶峰公司将领导这项应用，并与堪萨斯大学三位顶尖科学家组成的跨学科联盟合作。朱迪·吴教授在材料科学和纳米制造技术方面拥有丰富的经验，Mark Richter教授在蛋白质工程方面拥有熟练的技能，George Wilson教授为该项目带来了超过30年的生物传感器开发经验。顶峰公司现有的设施和设备以及堪萨斯大学的各种实验室将提供成功完成这一项目所需的资源。这一应用的创新方面包括使用活性酶功能化纳米颗粒作为传感元件，以及这些纳米颗粒的受控电泳沉积。同样新颖的是一种最大限度地提高氧气重复使用和过氧化氢检测效率的设计。这将导致线性微生物传感器具有高灵敏度的分析物被监测。在应用中给出了广泛的初步结果。这些结果是第一阶段工作的基础。 总体而言，2009财年全球生物传感器的销售额为69亿美元，其中31%的销售额来自人类葡萄糖传感器。顶峰公司是一家成熟的生物传感器制造商，能够很好地将这种新型生物传感器推向广阔的临床前市场。 公共卫生相关性：在美国，超过1000种大脑和神经系统疾病导致的住院人数比包括心脏病和癌症在内的任何其他疾病组都要多。与大脑和神经系统相关的疾病的生活质量、经济成本和成本都令人震惊。这些疾病每年扰乱5000多万美国人的生活，造成的损失超过4000亿美元。