Stem Cell and Pathology Core

干细胞和病理学核心将是这个PPG的关键资源。它将为四个项目提供广泛的试剂、程序和分析，包括i）CPC分离、分选和表征，ii）体外CPC功能测定，以及iii）体内获得的心脏样本的组织病理学。它将提供必要的设施和专业知识，以分离，维持和操纵来自几个基因工程小鼠的CPC。个别项目不具备所需的设施或专业知识，无法一致地分离和表征CPC，也无法进行广泛的组织病理学研究。 需要分析。将产品协调会的所有工作合并到一个核心设施中，将减少用品和设备的费用，因为核心将批量采购用品（从而减少开支），而且由于维持集中库存和盘存，将消除用品、试剂和设备的浪费和不必要的重复。将CPC工作合并为核心设施也是一项具有时间效益的工作，因为这项工作所涉及的技术是非常劳动密集型的，需要专门的熟练人员。核心工作人员是一个由六名专职人员组成的全职团队，由以下人员直接监督： 核心领导者将提供分析的一致性和可重复性。这是至关重要的，因为这将确保所有四个项目都将以相同的方式使用分离、扩展和排序的CPC，从而使结果具有可比性。单一来源的CPC具有严格的标准化，以确保一致性，这将导致在所有四个项目中移植统一的CPC群体，因此对于整合和比较每个项目的结果至关重要。同样，统一的组织病理学 由核心以盲法和严格标准化的方式进行的分析将使同一项目内和不同项目之间的不同实验结果得以比较。 核心用于制备CPC的方法非常有效;培养中的CPC， 通过c-kit表达（FACS）测定。第2 - 10代平均95 - 3%，第10代保持-95%。总而言之，核心小组将确保质量控制，并通过消除重复劳动和最大限度地利用人员和用品，使资源得到有效利用。

The Stem Cell and Pathology Core will be a critical resource to this PPG. It will provide the four Projects with access to a wide array of reagents, procedures, and analyses, including i) CPC isolation, sorting, and characterization, ii) assays of CPC function in vitro, and iii) histopathology of cardiac samples obtained in vivo. It will provide the facilities and expertise necessary to isolate, maintain, and manipulate CPCs from several genetically engineered mice. The individual Projects do not have the facilities or expertise needed to isolate and characterize CPCs consistently or to perform the extensive histopathological analyses required. Consolidation of all CPC work into a Core facilitv will decrease the costs of supplies and eguipment because the Core will make bulk purchases of supplies (thereby reducing expenses) and because waste and unnecessary duplication of supplies, reagents, and equipment will be eliminated with the maintenance of centralized stocks and inventories. Consolidating CPC work into a Core facilitv is also time-effective because the techniques involved in this work are very labor intensive and require dedicated, skilled personnel. The Core staff, a full-time team of six dedicated individuals under the immediate supervision of the Core Leaders, will provide consistency and reproducibility of analvsis. This is crucial, because it will ensure that all four Proiects will use CPCs isolated, expanded, and sorted the same way, thereby making the results comparable. A single source of CPCs with rigorous standardization to ensure consistency will result in transplantation of uniform CPC populations in all four Proiects and thus is extremely important for the integration and comparison of results from each of the Projects. Similarly, the uniform histopathologic analyses conducted by the Core in a blinded and rigorously standardized fashion will make it possible to compare results of different experiments within the same Project and among different Projects. The methods used by the Core to prepare CPCs are extremely efficient; CPCs in culture, as determined by c-kit expression (FACS). average 95¿3% at passages 2 to 10 and remain at -95% at passage 10. In summary, the Core will ensure guality control and, by eliminating duplication of effort and maximizing the use of personnel and supplies, will enable an efficient use of resources.