Task Specific Project 4

具体任务项目4：荧光淋巴结层析成像 在这个特定的任务项目#4中，我们将集成基于并行自适应有限元的层析成像 用于最大数据流的小型化FDPM测量设置的算法。我们将任务分解为 将近红外淋巴结层析成像转化为以下四个具体研究目标 拟议研究的第二年和第三年。 CT造影剂也可以获得组织模体的CT扫描图像，这将成为金标准 用于验证断层扫描结果的图像。将进行实验研究，以确定：(I)最低限度 准确的断层扫描定位所需的染料浓度，(Ii)两个结节状的最小距离 可以分辨目标，(Iii)可以重建的最小节点大小，以及(Iv)最佳的近红外激发 用于解析淋巴网络的照明图案。 特定任务#3验证用于淋巴结检测的断层扫描测量和算法 我们预计到第一年年底，移动摄影测量系统和软件将 可随时与小型化FDPM成像系统集成，用于临床LN断层扫描。我们将验证 猪的近红外荧光FDPM系统及其算法。在之前发表的研究中，来自杜克大学的研究人员。 塞维克的团队已经证明了成功成像和量化淋巴推进的能力 约克郡猪注射ID后的荧光染料[137]。在这个特定的目标下，近红外淋巴荧光 测量将通过在三个主要区域注射近红外染料来获得：(I)沿乳房链条 可视化淋巴流向腹股沟淋巴结，(Ii)在前后肢和后肢追踪流向 腹股沟、髂骨和髂下淋巴结，以及(Iii)在前肢和后肢追踪血流进入 腋窝和颈部。每只动物将分别进行两次皮内注射IDOil； 经过数小时的成像，动物将被实施安乐死。 在这一特定目标下的动物成像研究的目标将是确定最佳药剂剂量 用于高保真重建图像的集中度。注射造影剂的最佳浓度将是 通过对10只动物进行成像来确定，注射剂量从每只动物24微克近红外染料(即 相当于人类ICG的最大微剂量)到每只动物0.24|JG近红外染料。重建 图像分析将使用两个指标：(I)信噪比(SNR)和(Ii)淋巴管与正常组织相比的目标背景比(TBR)。

Task Specific Project #4: Fluorescence Lymph Node Tomography In this Specific Task Project #4, we will integrate the parallelized adaptive finite element based tomography algorithms to the miniaturized FDPM measurement setup for maximal data flow. We break down the task of translating NIR lymph node tomography into clinic into the following four specific research aims spread over years 2 and 3 of the proposed research. CT contrast agents as well to acquire CT scanned images of the tissue phantoms that will act as gold standard images for validating tomography results. Experimental studies will be carried out to determine: (i) minimal concentration of dye required for accurate tomographic localization, (ii) minimal distance at which two node-like targets can be resolved, (iii) minimal node size which can be reconstructed, and (iv) optimal NIR excitation illumination patterns for resolving lymphatic networks. Specific Task #3 Validate tomographic measurements and algorithms for lymph node detection in Swine We expect that by the end of the first year, the mobile photogrammetric system and software will be ready and integrated with the miniaturized FDPM imaging system for clinical LN tomography. We will validate the NIR fluorescence FDPM system and algorithms in swine. In previous published work, researchers from Dr. Sevick's group have demonstrated the ability to successfully image and quantify lymph propulsion of fluorescent dyes in Yorkshire swine after ID administration [137]. In this specific aim, NIR lymph fluorescence measurements will be acquired by injecting NIR dye in three major regions: (i) along the mammary chain to visualize lymphatics draining into inguinal nodes, (ii) in the anterior and posterior hind limbs for tracking flow to inguinal and iliac and sub-iliac lymph nodes, and (iii) in the anterior and posterior forelimbs to track flow into axilla and neck regions. Twleve intradermal injections of IDOiiL each will be performed per animal; after 6 hours of imaging, animals will be euthanized. The objective of animal imaging studies under this specific aim will be to determine an optimal agent dose concentration for high fidelity reconstructed images. The optimal concentration of injected contrast agents will be determined by imaging 10 animals with the injection dose decreasing from 24 ug NIR dye per animal (that corresponds to the maximum microdose of ICG in humans) to 0.24 |jg NIR dye per animal. Reconstructed images will be analyzed with two metrics: (i) signal to noise ratio (SNR), and (ii) target to background (TBR) of the lymphatics compared to normal tissue.