Capsaicin-sensitive primary afferent fibers and neuropathic pain

辣椒素敏感的初级传入纤维和神经性疼痛

• 批准号: 8282946
• 负责人: JENNIFER LYNN GIBBS
• 金额: $ 12.5万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8282946
• 关键词: Acute; Affect; Afferent Neurons; Age; Analgesics; Anesthetics; Animal Model; Animals; Area; Behavior; Behavioral; Biochemical; Biological Assay; Biological Markers; Biopsy; Capsaicin; Cell Culture Techniques; Classification; Clinical; Clinical Research; Cream; Cross-Sectional Studies; Data; Dermal; Detection; Development; Development Plans; Diagnosis; Disease Outbreaks; Doctor of Dental Surgery; Doctor of Philosophy; Endodontics; Evaluation; Fiber; Functional disorder; Herpes zoster disease; Herpesvirus Type 3; Home environment; Hyperalgesia; Image; Immunohistochemistry; Instruction; Laboratory Study; Lidocaine; Life; Measures; Mediating; Mentored Patient-Oriented Research Career Development Award; Modeling; Molecular; Neuroanatomy; Neurons; Neurosciences Research; Orofacial Pain; Outcome; Pain; Pain Research; Pain quality; Patients; Pattern; Peripheral; Peripheral nerve injury; Play; Population; Positioning Attribute; Postherpetic neuralgia; Principal Investigator; Quality of life; Rattus; Research; Research Personnel; Residencies; Role; Secondary to; Sensory; Series; Site; Skin; Symptoms; System; TRPV1 gene; Techniques; Testing; Tissues; Training; Translational Research; Trigeminal nerve structure; Work; allodynia; base; capsaicin receptor; career; central sensitization; clinically significant; cohort; experience; follow-up; inflammatory pain; insight; mechanical allodynia; nerve injury; nerve supply; neurochemistry; novel; orofacial; pain behavior; painful neuropathy; professor; relating to nervous system; research study; response; tool

Jennifer L. Gibbs, D.D.S., Ph.D. was appointed as an Assistant Adjunct Professor after completing her Endodontic Residency in July 2008. She has prior training in systems neuroscience research including biochemical assays, cell culture, anatomical studies, and behavioral experiments. This K-23 Mentored Patient-Oriented Research Career Development Award will be used to launch her career in translational orofacial pain research with a focus on understanding the mechanisms of neuropathic pain (NP). Research Plan: Dr. Gibbs has propsed a series of studies to evaluate the role of capsaicin-sensitive primary afferent neurons in mediating NP by performing a cross-sectional study on subjects diagnosed with post- herpetic neuralgia (PHN) and a complementary series of studies in animals using a model of orofacial NP. In these studies we will determine the relationship between a capsaicin response test (CRT) and outcomes of pain and sensory measures consistent with hyper-responsive primary afferents to determine if the CRT faciliatates a mechanism-based diagnosis of NP. In addition we will test the hjqjothesis that PHN subjects who respond to capsaicin have distinct epidermal and dermal innervation patterns and determine whether histological evaluation of skin biopsies can identify a biomarker for mechanism-based classification of NP. Finally, an animal model will be used to study the contribution of peripheral versus central terminals of capsaicin sensitive primary afferents in the setting of nerve injury Development Plan:Dr. Gibbs will continue her previously initiated work with Dr. Allan Basbaum in his laboratory studying basic mechanisms of pain and relevant techniques including neuroanatomy and pain behavior. In addition Dr. Gibbs will work with Dr. Rowbotham at the UCSF Pain Clinical Research Center to conduct sensory testing studies and collect biopsies from PHN subjects. Dr. Gibbs will continue collaborative efforts with Dr. Schmidt to identify biomarkers for NP and develop a new model of orofacial pain in animals. Dr. Gibbs is also currently pursuing a Master's in Clinical Reseach to facilitate her ability to perform translational research. Dr. Gibbs has the full support ofher home department and will transition to a tenure track position in July 2009. RELEVANCE (See instructions): These studies will yield novel and important insights into the mechanisms contributing th NP and help define populations (e.g. capsaicin responders) who are more likely to benefit from mechanims targeting analgesic therapies. Neuropathic pain has devastating impact on patients ability to inderact socially, work, and overall enjoyment of life. Improvement in diagnosis of NP is critical for better management of these patients.

詹妮弗·L·吉布斯博士在完成牙髓学课程后被任命为助理教授 2008年7月入驻。她之前接受过系统神经科学研究方面的培训，包括生化分析， 细胞培养、解剖学研究和行为实验。这位K-23指导了以病人为中心的研究 职业发展奖将用于启动她在翻译口面部疼痛研究方面的职业生涯，重点是 关于神经病理性疼痛(NP)机制的理解。 研究计划：吉布斯博士提出了一系列研究，以评估辣椒素敏感的初级辣椒素的作用 通过对诊断为后遗症的受试者进行横断面研究，研究传入神经元在调节NP中的作用 疱疹病毒性神经痛(PHN)和一系列使用口腔面部NP模型进行的动物补充研究。在……里面 这些研究我们将确定辣椒素反应试验(CRT)和疼痛结局之间的关系 以及与高反应初级传入相一致的感觉测量，以确定CRT是否使 基于机理的NP诊断。此外，我们将测试对PHN受试者有反应的HJQ关节 辣椒素具有不同的表皮和真皮神经支配模式，并决定组织学 皮肤活检的评估可以确定一个基于机制的NP分类的生物标记物。最后，一只动物 模型将被用来研究辣椒素敏感初级的外周终末和中央终末的贡献 神经损伤环境中的传入 发展计划：吉布斯博士将在他的实验室继续她之前与艾伦·巴斯鲍姆博士一起发起的工作 研究疼痛的基本机制和相关技术，包括神经解剖学和疼痛行为。在……里面 此外，吉布斯博士将在加州大学旧金山分校疼痛临床研究中心与罗伯坦博士合作，进行感官 测试研究并收集PHN受试者的活组织检查。吉布斯博士将继续与吉布斯博士合作。 施密特为NP确定生物标记物，并在动物身上开发一种新的口面部疼痛模型。吉布斯博士也是 目前正在攻读临床研究硕士学位，以促进她进行翻译研究的能力。Dr。 吉布斯得到了内政部的全力支持，将于2009年7月过渡到终身教职。 相关性(请参阅说明)： 这些研究将对THNP的形成机制产生新的和重要的见解，并有助于确定 更有可能受益于针对止痛剂的机制的人群(例如辣椒素应答者) 治疗。神经性疼痛对患者的社交能力、工作能力和整体能力具有毁灭性的影响 享受生活。提高NP的诊断水平是更好地处理这些患者的关键。