Oxidant Stress Mechanisms in Preeclampsia

先兆子痫的氧化应激机制

• 批准号: 8207197
• 负责人: SCOTT W WALSH
• 金额: $ 36.29万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-05 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8207197
• 关键词: Abdomen; Accounting; Angiotensin II; Antibodies; Antioxidants; Arteries; Blood Pressure; Blood Vessels; Candidate Disease Gene; Cardiovascular Diseases; Cardiovascular Physiology; Cell Adhesion Molecules; Cell Culture Techniques; Cells; Cesarean section; Chemistry; Chronic; Coculture Techniques; DNA Methylation; DNA Sequence Analysis; Endothelium; Enzyme-Linked Immunosorbent Assay; Enzymes; Epigenetic Process; Extracellular Matrix; Fatty acid glycerol esters; Functional disorder; Future; Genes; Health; Human; Immunohistochemistry; In Situ; Inflammation; Inflammatory; Inflammatory Response; Intercellular adhesion molecule 1; Interleukin-8; Interstitial Collagenase; Life; Long-Term Effects; Measures; Mediating; Methylation; Modeling; Molecular; Morbidity - disease rate; Neutrophil Activation; Neutrophil Collagenase; Neutrophil Infiltration; Norepinephrine; Nuclear; Omentum; Operative Surgical Procedures; Oxidative Stress; Pathology; Patients; Pattern; Peroxidases; Phenotype; Phosphorylation; Phosphotransferases; Plasma; Platelet Activation; Poison; Pre-Eclampsia; Pregnancy; Production; Reactive Oxygen Species; Recurrence; Reporter; Research; Risk; Role; Smooth Muscle Myocytes; System; TNF gene; Testing; Thromboxane Production; Thromboxanes; Time; Tissue Stains; Tissues; Transfection; Tumor Necrosis Factor-alpha; Two-Dimensional Gel Electrophoresis; Vascular Smooth Muscle; Vascular System; Vasoconstrictor Agents; Western Blotting; Woman; bisulfite; cardiovascular disorder risk; chemokine; cyclooxygenase 2; cytokine; fetal; imprint; inflammatory marker; inhibitor/antagonist; mortality; mutant; myosin phosphatase; neutralizing antibody; neutrophil; novel; oxidant stress; pregnant; programs; public health relevance; response; subcutaneous; vascular inflammation; vasoconstriction

DESCRIPTION (provided by applicant): The over-arching hypothesis to be tested in this research is that vascular inflammation due to neutrophil infiltration is critical to the pathophysiology of preeclampsia. Neutrophils infiltrating the vasculature could cause inflammation and vasoconstriction by release of toxic compounds, such as reactive oxygen species (ROS), TNF??and thromboxane. In Specific Aim 1, we will determine if maternal vascular infiltration by neutrophils is a hallmark of preeclampsia, and associated with vascular inflammation. Subcutaneous fat, omental fat and placental tissue from pregnant patients will be collected and evaluated by immunohisto chemistry to assess vascular phenotypes of normal nonpregnant, normal pregnant and preeclamptic women, and to determine if neutrophil infiltration is restricted to the maternal compartment. We will stain tissues for CD66b (a neutrophil marker), intercellular adhesion molecule-1 (an endothelial adhesion molecule for neutrophils), interleukin-8 (IL-8, a potent neutrophil chemokine), NF-?B and COX-2 (hallmarks of inflammation), myeloperoxidase (a potent oxidizing enzyme), and matrix metalloproteinases 1 and 8. The in situ findings of vascular pathology observed in this Aim will drive the mechanistic studies of Aims 2-4 which will examine mechanisms for neutrophil induced vascular inflammation and vasoconstriction. In Specific Aim 2, we will evaluate whether neutrophils that have infiltrated into the intimal space could be responsible for transmitting inflammation to the vascular smooth muscle by co-culturing neutrophils with human vascular smooth cells. We will use transfection and cell culture to determine if neutrophils can activate NF-?B and induce expression of COX-2, IL-8 and thromboxane in human vascular smooth muscle cells (VSMC). In Specific Aim 3, we will use a myograph system with isolated omental vessels and cell culture of VSMC to examine cellular mechanisms whereby neutrophils could cause vasoconstriction and/or enhance vessel reactivity to vasoconstrictors. In Specific Aim 4, we will determine if neutrophils or neutrophil products induce epigenetic changes in vascular smooth muscle genes related to inflammation, and thus, may program adverse long-term effects on maternal cardiovascular function. Methylation patterns of candidate genes will be evaluated in vessels from women with normal pregnancies and women with preeclampsia by bisulfite DNA sequence analysis and COBRA (Combined Bisulfite Restriction Analysis). We will also determine if neutrophils or neutrophil products (ROS, TNF?) alter the methylation status of these genes in a similar pattern in cultured VSMC. This novel hypothesis may explain future health issues, including recurrent preeclampsia and future risk of cardiovascular disease. PUBLIC HEALTH RELEVANCE: Preeclampsia is a leading cause of maternal and fetal morbidity and mortality. This research will test the hypothesis that vascular inflammation due to neutrophil infiltration is critical to the pathophysiology of preeclampsia. This hypothesis encompasses molecular explanations for a vicious cycle that reinforces vascular inflammation in preeclampsia, and creates an epigenetic imprint in the maternal vascular system that may predispose women to chronic cardiovascular disease.

描述（由申请人提供）：本研究中要检验的过度假设是，中性粒细胞浸润引起的血管炎症对先兆子痫的病理生理学至关重要。浸润血管的中性粒细胞可通过释放毒性化合物，如活性氧（ROS）、TNF？和血栓素。在具体目标1中，我们将确定母体血管中性粒细胞浸润是否是先兆子痫的标志，并与血管炎症相关。将采集妊娠患者的皮下脂肪、网膜脂肪和胎盘组织，并通过免疫组织化学进行评价，以评估正常非妊娠、正常妊娠和先兆子痫女性的血管表型，并确定中性粒细胞浸润是否仅限于母体室。我们将对组织进行CD 66 b（一种中性粒细胞标记物）、细胞间粘附分子-1（一种中性粒细胞内皮粘附分子）、白细胞介素-8（IL-8，一种有效的中性粒细胞趋化因子）、NF-？B和考克斯-2（炎症标志）、髓过氧化物酶（一种有效的氧化酶）和基质金属蛋白酶1和8。在本目标中观察到的血管病理学的原位发现将推动目标2-4的机制研究，其将检查中性粒细胞诱导的血管炎症和血管收缩的机制。在特定目标2中，我们将通过将中性粒细胞与人血管平滑肌细胞共培养，评价浸润到内膜空间的中性粒细胞是否负责将炎症传递到血管平滑肌。我们将使用转染和细胞培养，以确定是否中性粒细胞可以激活NF-？B诱导人血管平滑肌细胞（VSMC）表达考克斯-2、IL-8和血栓素。在特定目标3中，我们将使用具有分离的网膜血管和VSMC细胞培养物的肌描记系统来检查中性粒细胞可引起血管收缩和/或增强血管对血管收缩剂的反应性的细胞机制。在具体目标4中，我们将确定中性粒细胞或中性粒细胞产物是否诱导与炎症相关的血管平滑肌基因的表观遗传变化，从而可能对母体心血管功能产生长期不良影响。将通过亚硫酸氢盐DNA序列分析和COBRA（联合亚硫酸氢盐限制性分析）在正常妊娠女性和先兆子痫女性的血管中评价候选基因的甲基化模式。我们还将确定中性粒细胞或中性粒细胞产物（ROS，TNF？）在培养的VSMC中以类似的模式改变这些基因的甲基化状态。这一新的假说可以解释未来的健康问题，包括复发性先兆子痫和未来心血管疾病的风险。 公共卫生相关性：先兆子痫是孕产妇和胎儿发病率和死亡率的主要原因。本研究将验证中性粒细胞浸润引起的血管炎症对先兆子痫的病理生理学至关重要的假设。这一假说涵盖了对恶性循环的分子解释，恶性循环加强了先兆子痫的血管炎症，并在母体血管系统中产生了表观遗传印记，可能使女性易患慢性心血管疾病。