ASSAY CORE

检测核心

• 批准号: 8231131
• 负责人: KRISTIN J BERGSLAND
• 金额: $ 22.92万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2016-07-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8231131
• 关键词: Aliquot; Bicarbonates; Biological Assay; Biopsy; Blood; Calcitriol; Calcium; Calcium Oxalate; Calculi; Chemistry; Chlorthalidone; Clinical Research; Cyclic AMP; Data; Databases; Defect; Deposition; Distal; Diuresis; Dose; Duct (organ) structure; Eating; Excretory function; Fasting; Furosemide; Future; Glucagon; Histology; Hormones; Instruction; Insulin; Kidney; Laboratories; Lead; Limb structure; Lithium; Maps; Measurement; Measures; Metabolic; Minerals; Nephrolithiasis; Operative Surgical Procedures; Oxalates; Papillary; Parathyroid gland; Pathogenesis; Patients; Plasma; Process; Protocols documentation; Renal tubule structure; Renin; Risk; Risk Factors; Sampling; Security; Serum; Shipping; Ships; Sodium; Testing; Thiazide Diuretics; Thick; Time; Tissues; Urine; Vitamin D; Water; absorption; calcification; calcium phosphate; feeding; glucagon-like peptide 1; hypercalciuria; metabolic abnormality assessment; programs

Core A measures serum, serum ultrafiltrate (UF), plasma and urine stone risk chemistries and calculates urine supersaturations (SS) for patients studied in Projects 1 and 2. These measurements are used for fulfillment of aims within all three projects and six program aims carried out within the overall program project. Plasma GLP-1, renin, and plasma and urine cAMP will also be measured by Core A for Project 1. Blood and urine samples are processed and shipped for assay of lithium done in Core B and for measurement of plasma glucagon, parathyroid hormone (PTH) and 1,25 vitamin D (performed by LabCorp). Sample aliquots are stored at -80¿C for future use. All laboratory data from Core A for all projects are consolidated into a single database with daily backup for security and ease of analysis. Ultimately, data from the other projects and cores (e.g. histology, biopsy, stone analysis etc.) will be consolidated into our database so that it can be analyzed along with serum and urine data.

核心 A 测量项目 1 和 2 中研究的患者的血清、血清超滤液 (UF)、血浆和尿石风险化学成分，并计算尿液过饱和度 (SS)。这些测量结果用于实现所有三个项目的目标以及整个计划项目中执行的六个计划目标。项目 1 的核心 A 还将测量血浆 GLP-1、肾素以及血浆和尿液 cAMP。 血液和尿液样本经过处理和运输，用于在 Core B 中进行锂测定，并测量血浆胰高血糖素、甲状旁腺激素 (PTH) 和 1,25 维生素 D（由 LabCorp 执行）。 样品等分试样储存在-80°C 下以供将来使用。所有项目的 Core A 的所有实验室数据都整合到一个数据库中，并每日备份，以确保安全性和易于分析。最终，来自其他项目和核心的数据（例如组织学、活检、结石分析等）将合并到我们的数据中 数据库，以便可以与血清和尿液数据一起进行分析。