Assay Core

检测核心

• 批准号: 9359528
• 负责人: KRISTIN J BERGSLAND
• 金额: $ 21.98万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9359528
• 关键词: Achievement; Affect; Aldosterone; Aliquot; Biological Assay; Biological Markers; Biopsy; Blood; Body Size; CLDN14 gene; Calcified; Calcium; Calcium Oxalate; Carrier Proteins; Chemistry; Child; Cimetidine; Clinical; Clinical Research; Collection; Creatinine; Crystallization; Data; Databases; Diet; Dihydroxycholecalciferols; Disease; Dissociation; Distal; Enzyme-Linked Immunosorbent Assay; Future; Histology; Histopathology; Impaired Renal Function; Inflammation; Inflammatory Response; Injury; Ions; Kidney; Kidney Diseases; Laboratories; Lead; Link; Lithium; Measurement; Measures; Metabolic; Methods; Minerals; Molecular; Nephrolithiasis; Nephrons; Outcome; PTH gene; Papillary; Pathogenesis; Patients; Physiological; Physiology; Plasma; Process; Property; Protocols documentation; Regulation; Renin; Risk; Sampling; Security; Serum; Ships; Sodium; Specimen; Testing; Time; Tissues; Tubular formation; Urine; Variant; Vitamin D; apical membrane; calcification; calcium excretion; calcium phosphate; disease phenotype; exosome; functional disability; hypercalciuria; inflammatory marker; potassium citrate; programs; renal calcium; response; solute; urinary

Summary Core A measures serum, serum ultrafiltrate, plasma and urine stone risk chemistries and calculates urine supersaturations for patients studied in Projects 1 and 2. These measurements are used for fulfillment of aims within all four central program project objectives. Upper limit of metastability (ULM) for calcium oxalate and calcium phosphate in urine will be measured for Project 1. Core A will also measure plasma renin for Project 1. Blood and urine samples are processed and shipped for assay of lithium done in Core B, measurement of inflammatory markers in Core D and measurement of intact parathyroid hormone (PTH), aldosterone, 25(OH)- vitamin D and 1,25-dihydroxyvitamin D (performed by LabCorp). Urine exosomes will be prepared for molecular expression studies of renal ion transporter proteins. Aliquots of all blood and urine specimens are stored at -80°C for future use. All laboratory data from Core A for all projects are consolidated into a single database with daily backup for security and ease of analysis. Ultimately, data from the other projects and cores (e.g. histology, biopsy, stone analysis etc.) will be consolidated into our database so that it can be analyzed along with serum and urine data.

概括 核心 A 测量血清、血清超滤液、血浆和尿液结石风险化学成分并计算尿液 项目 1 和 2 中研究的患者的过饱和度。这些测量用于实现目标 在所有四个中心计划项目目标之内。草酸钙和亚稳定性上限 (ULM) 项目 1 将测量尿液中的磷酸钙。核心 A 还将测量项目 1 的血浆肾素。 血液和尿液样本经过处理和运输，用于在核心 B 中进行锂测定，测量 Core D 中的炎症标记物以及完整甲状旁腺激素 (PTH)、醛固酮、25(OH)- 的测量 维生素 D 和 1,25-二羟基维生素 D（由 LabCorp 进行）。尿液外泌体将被制备用于 肾离子转运蛋白的分子表达研究。所有血液和尿液样本的等分试样 储存于-80°C以备将来使用。所有项目的核心 A 的所有实验室数据都合并为一个 数据库每日备份，以确保安全性并易于分析。最终，来自其他项目和核心的数据 （例如组织学、活检、结石分析等）将被合并到我们的数据库中，以便进行分析 以及血清和尿液数据。