Cell Cuture Core

这个蜂窝核心已经连续为该计划项目服务了25年，拥有所有必要的基础设施、设备、空间和人员。汉森博士首创了人类血细胞分离程序，目前仍在使用，以提供白细胞。布拉顿博士多年来一直负责这一资源的血细胞分离和监管管理。莱斯利博士和她的实验室提供了一致的、持续的巨噬细胞分离和细胞培养。我们的合作者罗伯特·梅森博士(见Letter)向细胞核心提供的人肺泡巨噬细胞将被培养并分配给项目。提供的细胞来源是四个：1)分离的人血细胞，2)人肺泡巨噬细胞，3)来自肺和腹膜的常驻巨噬细胞和来自基因敲除和野生型小鼠的用于巨噬细胞来源的骨髓细胞，以及4)培养细胞系。描述了细胞分离和培养的简单方法，并包括了详细说明所用程序的参考清单。资源和环境一节概述了专用空间、设备和必要资源的说明。该单元核心的预算理由反映了将为两个或更多项目共同使用的服务和资源。

This CELL CORE has continuously served this Program Project for 25 years and has all necessary infrastructure, equipment, space and personnel. Dr. Henson originated the human blood cell isolation procedures that are still in use for the provision of leukocytes. Dr. Bratton has overseen the blood cell isolation and regulatory management of this resource for many years now. Dr. Leslie and her laboratory have provided consistent, continuous macrophage isolation and cell culture. Human alveolar macrophages provided by our collaborator Dr. Robert Mason (see letter) to the CELL CORE will be cultured and distributed to the Projects. Sources of the provided cells are four: 1) isolated human blood cells, 2) human alveolar macrophages, 3) resident macrophages from the lungs and peritonea and bone marrow cells for macrophage derivation from knockout and wild type mice, and 4) cultured cell lines. Brief methods for cell isolation and culture are described and a reference list detailing procedures to be utilized is included. The description of dedicated space, equipment and necessary resources is outlined in the Resources and Environment section. The Budget Justification for the CELL CORE reflects services and resources to be utilized in common for two or more Projects.