Cell Cuture Core

该CELL CORE已连续服务于该项目25年，并拥有所有必要的基础设施、设备、空间和人员。汉森博士创立了人类血细胞分离程序，目前仍用于提供白细胞。Bratton博士多年来一直监督血细胞分离和这种资源的监管管理。Leslie博士和她的实验室提供了一致的、连续的巨噬细胞分离和细胞培养。我们的合作者Robert Mason博士（见信）提供给CELL CORE的人类肺泡巨噬细胞将被培养并分发给项目。提供的细胞来源有四种：1)分离的人血细胞，2)人肺泡巨噬细胞，3)来自肺和腹膜的常驻巨噬细胞，以及来自敲除和野生型小鼠的巨噬细胞衍生的骨髓细胞，4)培养的细胞系。简述了细胞分离和培养的方法，并提供了一份详细说明使用程序的参考清单。资源和环境部分概述了专用空间、设备和必要资源的描述。CELL CORE的预算理由反映了两个或多个项目共同使用的服务和资源。

This CELL CORE has continuously served this Program Project for 25 years and has all necessary infrastructure, equipment, space and personnel. Dr. Henson originated the human blood cell isolation procedures that are still in use for the provision of leukocytes. Dr. Bratton has overseen the blood cell isolation and regulatory management of this resource for many years now. Dr. Leslie and her laboratory have provided consistent, continuous macrophage isolation and cell culture. Human alveolar macrophages provided by our collaborator Dr. Robert Mason (see letter) to the CELL CORE will be cultured and distributed to the Projects. Sources of the provided cells are four: 1) isolated human blood cells, 2) human alveolar macrophages, 3) resident macrophages from the lungs and peritonea and bone marrow cells for macrophage derivation from knockout and wild type mice, and 4) cultured cell lines. Brief methods for cell isolation and culture are described and a reference list detailing procedures to be utilized is included. The description of dedicated space, equipment and necessary resources is outlined in the Resources and Environment section. The Budget Justification for the CELL CORE reflects services and resources to be utilized in common for two or more Projects.