Genetic Engineering Mouse Core

基因工程小鼠(GEM)品系的构建和表型分析是基础 对于胰腺癌的研究，包括对信号分子的分析，是不可或缺的 病理学，新基因及其关联网络的发现和分析，以及验证和 评估新的治疗靶点和相关的分子生物标记物。基因工程 此PDAC POI的鼠标核心(GEMC)将提供所有必要的专业知识、试剂和服务 在每年的赠款资金中产生四个转基因小鼠(GEM)建模项目。GEMC 将与POI项目负责人、研究人员密切合作，生产最先进的癌症相关宝石 菌株。具体地说，就是： (A)转基因；GEMC将支持构建转基因小鼠模型的所有方面， 包括为每个具体项目的最佳设计和施工提供建议、服务、技术和试剂 转基因。 (B)基因打靶；我们将支持构建敲除和敲入小鼠等位基因的所有方面， 包括为优化设计、施工提供服务、支持、建议、技术和试剂 以及生产每个特定的靶向载体和所产生的小鼠。 (C)评估和实施构建基因工程小鼠的新技术，并 派生细胞。

The construction and phenotypic analysis of genetically engineered mouse (GEM) strains are fundamental and integral for the study of pancreatic cancer, including the analysis of signaling molecules in this pathology, the discovery and analysis of novel genes and their linked networks, and the validation and assessment of novel therapeutic targets and associated molecular biomarkers. The Genetic Engineering Mouse Core (GEMC) of this PDAC POI will provide all the necessary expertise, reagents and services to generate four genetically engineered mouse (GEM) modeling projects per year of grant funding. The GEMC will work closely with POI project leaders, investigators to produce the most advanced cancer relevant GEM strains. Specifically: (A) Transgenics; the GEMC will support all aspects of the construction of transgenic mouse models, including advice, service, technologies and reagents for the optimal design and construction of each specific transgene. (B) Gene Targeting; We will support all aspects of the construction of knockout and knock-in mouse alleles, including provide services, support, advice, technologies and reagents for the optimal design, construction and production of each specific targeting vector and resulting mice. (C) Evaluate and implement new technologies for the construction of genetically engineered mice and derivative cells.