Flow Cytometry

流式细胞术/细胞分选是当代免疫学和细胞生物学研究的重要资源。 该计划内的所有项目和Core 3都对该技术有广泛的需求，其中包括 病例，需要相同的应用和试剂。有鉴于此，我们将这些需求整合到了Core中 一是实现规模经济。核心A将提供流式细胞仪和细胞分选需求的方案 参与者。这将包括基于最多七个参数(光散射和 荧光)和罕见事件分析。该设施还将提供多达9个电池参数分析 表面标记表达(免疫荧光)和各种静态细胞生理参数(细胞凋亡， 细胞活力和细胞周期)，以及实时细胞生理变化(钙动员、细胞内 易位和线粒体膜电位等。)在附加参数上设置门控。服务将 使用目前组成国家犹太医疗和医疗中心的七个设施工具提供 研究中心机构的流式细胞仪设备。

Flow cytometry/cell sorting is a critical resource for contemporary research in immunology and cell biology. All projects within this program, and Core 3, have extensive needs for this technology including, in some cases, needs for the same application and reagents. In view of this, we have integrated these needs in Core A to accomplish economy of scale. Core A will provide for flow cytometry and cell sorting needs of program participants. This will include high rate cell sorting based on up to seven parameters (light scatter and fluorescence) and rare event analysis. The facility will also provide up to nine parameter analysis of cell surface marker expression (immunofluorescence) and various static cell physiologic parameters (apoptosis, cell viability, and cell cycle), as well as real-time cell physiologic changes (calcium mobilization, intracellular translocation and mitochondrial membrane potential, etc.) with gating on additional parameters. Services will be provided using the seven facility instruments that currently constitute the National Jewish Medical and Research Center institutional flow cytometry facility.