ANIMAL MODELS OF REDOX METABOLISM AND ARTERIAL DYSFUNCTION

氧化还原代谢和动脉功能障碍的动物模型

• 批准号: 8250450
• 负责人: Thomas Michel
• 金额: $ 22.13万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8250450
• 关键词: Animal Model; Biochemical; Biochemistry; Biological Markers; Blood Vessels; Clinical; Collaborations; Consult; Data; Endothelial Cells; Experimental Designs; Functional disorder; Individual; Knockout Mice; Letters; Metabolic; Metabolism; Mus; Oxidation-Reduction; Pharmacology; Phenotype; Physiological; Physiology; Preparation; Research Personnel; Transgenic Mice; experience; experimental analysis; in vivo; mouse model; programs; research study

Overview and Specific Aims Core B will provide support for all four Projects by 1) performing vascular physiology and pharmacology experiments in the transgenic and knockout mouse models being investigated in this PPG, and 2) by coordinating the proposed studies with the Yale Mouse Metabolic Phenotyping Center (YMMC) for the detailed in vivo metabolic characterizations that are planned for several ofthe mouse models being studied in this Program. The proposed studies of mouse vascular physiology and metabolism being supported in this Core will provide a critical context for the data that will be generated by Program's proposed Common Experiment, which explores redox biochemistry and redox biomarkers in mouse endothelial cells. Dr. Michel, who has served as Director of the Animal Models of Arterial Dysfunction Core Lab during the current project period, is an experienced vascular biologist who has been involved in productive collaborations studying murine vascular physiology and pharmacology with the pther Project Leaders. Dr. Gerald Shulman (see attached letter), a leading investigator in metabolism, is the director of the YMMC, and is already collaborating with Drs. Plutzky and Lee; Dr. Shulman will continue to consult on experimental design and analysis in the proposed studies, which are described in detail in all the individual Project descriptions above.

概述和具体目标 核心B将通过以下方式为所有四个项目提供支持：1）在本PPG中研究的转基因和基因敲除小鼠模型中进行血管生理学和药理学实验; 2）与耶鲁小鼠代谢表型分析中心（YMMC）协调拟定研究，以便对本项目中研究的几种小鼠模型进行详细的体内代谢表征。本核心中支持的小鼠血管生理学和代谢的拟议研究将为该计划拟议的共同实验产生的数据提供关键背景，该实验探索小鼠内皮细胞中的氧化还原生物化学和氧化还原生物标志物。Michel博士在当前项目期间担任动脉功能障碍动物模型核心实验室主任，是一位经验丰富的血管生物学家，曾与pther项目负责人进行过富有成效的合作，研究小鼠血管生理学和药理学。Gerald Shulman博士（见所附信函）是代谢领域的主要研究者，是YMMC的负责人，他已经与Plutzky博士和Lee博士合作; Shulman博士将继续就拟议研究中的实验设计和分析提供咨询，这些研究在上述所有单个项目描述中有详细描述。